AI Article Synopsis
- Developed a zebrafish teratogenicity assay that accurately identifies teratogens with 87% concordance, using dechorionated AB strain embryos.
- The assay employs a morphological scoring system to assess adverse effects and determine the no-observed-adverse-effect level (NOAEL).
- The scoring system evaluates various structures and organ systems, providing insights into tissue-specific teratogenicity and allowing for ranking of compounds based on the severity of malformations.
Article Abstract
Background: Recently we reported the development and optimization of a zebrafish teratogenicity assay using dechorionated AB strain embryos, a promising assay that was 87% concordant in correctly identifying in vivo teratogens and non-teratogens from a set of 31 compounds (Brannen et al., 2010: Birth Defects Res 89:66-77).
Methods: This assay utilizes a zebrafish morphological score system to characterize adverse effects and identify the no-observed-adverse-effect level (NOAEL).
Results: This report describes in detail the morphological score system used in the dechorionated zebrafish embryo culture teratogenicity assay. The morphological assessment includes evaluation of most structures and organ systems and grades relative severity of abnormalities.
Conclusions: To this end, the morphological score system provides information of tissue-specific teratogenicity that has been found to have good concordance with structures found affected in vivo and can also be used to rank compounds based on the severity of malformations.
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| http://dx.doi.org/10.1002/bdrb.20260 | DOI Listing |
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