We report the application of pressure perturbation calorimetry (PPC) to study unfolded proteins. Using PPC we have measured the temperature dependence of the thermal expansion coefficient, α(T), in the unfolded state of apocytochrome C and reduced BPTI. We have shown that α(T) is a nonlinear function and decreases with increasing temperature. The decrease is most significant in the low (2-55 °C) temperature range. We have also tested an empirical additivity approach to predict α(T) of unfolded state from the amino acid sequence using α(T) values for individual amino acids. A comparison of the experimental and calculated functions shows a very good agreement, both in absolute values of α(T) and in its temperature dependence. Such an agreement suggests the applicability of using empirical calculations to predict α(T) of any unfolded protein.
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Article Synopsis
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- Researchers developed a model using induced pluripotent stem cells (iPSCs) to study the effects of having one Z allele on liver cells (hepatocytes).
- Findings show that these heterozygous cells have an intermediate disease phenotype and experience issues with protein processing and cellular function, similar to those with two Z alleles, indicating that just one Z allele can disrupt liver cell function.
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