Pseudo-outbreak of adenovirus infection in a neonatal intensive care unit due to a false-positive antigen detection test.

J Clin Microbiol

Department of Pediatrics, Division of Infectious Diseases, State University of New York at Buffalo School of Medicine and Biomedical Sciences, and the Women and Children’s Hospital of Buffalo, Buffalo, New York 14222, USA.

Published: November 2010

AI Article Synopsis

  • In a study of 56 infants in a neonatal intensive care unit, 28 tested positive for adenovirus using the Sure-Vue antigen test.
  • However, subsequent virus cultures and PCR tests on stool samples did not detect the virus, indicating a discrepancy in results.
  • The researchers were unable to identify the reason for the 50% false-positive rate of the antigen test.

Article Abstract

Twenty-eight of 56 infants in a neonatal intensive care unit had stools positive for adenovirus by the Sure-Vue adenovirus test. Virus cultures of conventionally processed and chloroform-extracted stool samples, as well as conventional and real-time PCR tests, were negative for adenovirus. The cause for the 50% false-positive rate with the antigen test was not determined.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020892PMC
http://dx.doi.org/10.1128/JCM.01262-10DOI Listing

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