AI Article Synopsis
- Researchers isolated plasmids from two bacterial strains, Paracoccus sp. BW001 and Shinella zoogloeoides BC026, to study their characteristics and connection to biodegradation.
- Analysis revealed BW001 has two large plasmids (190-245 kb) and one small plasmid (4.5-5.0 kb), while BC026 contains at least three large plasmids over 200 kb.
- Plasmid curing experiments suggested that biodegradation genes might be located on these plasmids, and transferring BW001's plasmids to E. coli 5alpha enabled the new strain to tolerate pyridine.
Article Abstract
In order to identify the characteristics of the plasmids of degrading bacterial strains and the relationship between the plasmids' function and biodegradation, plasmids were isolated from two bacterial strains (Paracoccus sp. BW001 and Shinella zoogloeoides BC026) and pulse-field gel electrophoresis was used to identify the distribution of plasmids and their molecular size. Two large plasmids with 190-245 kb and one small plasmid with 4.5-5.0 kb were found in the BW001, and at least 3 large plasmids over 200 kb were harbored in the BC026. The plasmid curing was conducted by high temperature-SDS method and the results indicated the biodegradation genes might locate in the plasmids of two bacterial strains. After transforming the plasmids of BW001 into E. coli 5alpha by electroporation, the new bacterial strain could tolerate pyridine.
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