AI Article Synopsis
- Heterosis refers to the increased performance of first-generation offspring compared to their parents, highlighting the limitations of traditional breeding methods.
- Researchers developed a transgenic system to create male sterile plants by targeting a specific gene (CP4 EPSPS) to prevent male reproductive tissue from tolerating glyphosate, while the rest of the plant remains resistant.
- This inducible male sterility system is user-friendly and shows potential for broad application in various crop plants.
Article Abstract
Background: Heterosis is a phenomenon that first-generation offspring perform better than their parents. Conventional breeding methods have their shortcomings. It would be optimal to construct inducible male sterile plants.
Results: We developed a novel system for creating male sterile transgenic plants by downregulating the specific expression of the glyphosate tolerance CP4 EPSPS gene in male reproductive tissues. Transcriptional repression was achieved by manipulating DNA binding proteins with their specific corresponding sites. We transferred the CP4 EPSPS gene driven by a modified CaMV 35S promoter with three tetracycline operator copies in the vicinity of the TATA box and tetracycline repressor gene under the control of an anther-specific promoter Osg6B to Arabidopsis thaliana. As a result, we successfully obtained controllable transgenic plants: the whole plant could tolerate exposure of glyphosate but the male tissue was sensitive.
Conclusion: The novel inducible male sterility system is applied and easy to handle, so it might be applicable to a wide range of crop plants.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jsfa.4117 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!