AI Article Synopsis
- An enzyme immunoassay (EIA) was developed using nitrocellulose paper discs coated with testosterone-specific antibodies to measure serum testosterone levels.
- The assay's effectiveness was validated against a microtitre plate-based ELISA and a commercial radioimmunoassay (RIA), showing a strong correlation (0.84) and minimal cross-reactivity with other steroids.
- The method demonstrated low variation in results, is convenient for transport and storage, and is less expensive compared to existing methods.
Article Abstract
An enzyme immunoassay (EIA) for the measurement of serum testosterone has been developed using nitrocellulose paper discs as the solid support. The paper discs (6 mm diameter) coated with testosterone-specific antibody were incubated with testosterone and testosterone-peroxidase conjugate in glass tubes. The amount of testosterone present in samples could be estimated from the bound peroxidase activity. The assay was validated by comparison with a microtitre plate-based ELISA and a commercial radioimmunoassay (RIA) kit. The correlation coefficient between RIA and EIA was 0.84. No significant cross reactivity was observed with other steroids, except for dihydrotestosterone. The inter- and intra-assay coefficients of variation were 4.4 and 9.6% respectively. The preservation and transport of the coated paper discs are convenient and the overall cost of the method is less than that of other methods.
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| http://dx.doi.org/10.1515/cclm.1990.28.12.943 | DOI Listing |
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