Improved method for bacterial cell capture after flow cytometry cell sorting.

Appl Environ Microbiol

UPMC Université Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66651 Banyuls/mer, France.

Published: November 2010

Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2976227PMC
http://dx.doi.org/10.1128/AEM.00621-10DOI Listing

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