Quantitative real-time polymerase chain reaction (qRT-PCR) is a flexible and scalable method for analyzing transcript abundance that can be used at a single gene or high-throughput (>100 genes) level. Information obtained from this technique can be used as an indicator of potential regulation of glycosylation at the transcript level when combined with glycan structural or protein abundance data. This chapter describes detailed methods to design and perform qRT-PCR analyses and provides examples of information that can be obtained from the technique.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104320 | PMC |
| http://dx.doi.org/10.1016/S0076-6879(10)79004-2 | DOI Listing |
Publication Analysis
Top Keywords
transcript analysis
4
analysis stem
4
stem cells
4
cells quantitative
4
quantitative real-time
4
real-time polymerase
4
polymerase chain
4
chain reaction
4
reaction qrt-pcr
4
qrt-pcr flexible
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!