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Synthesis of uniform Pickering microspheres doped with quantum dot by microfluidic technology and its application in tumor marker.
Talanta
September 2023
Department of Health Technology, Technical University of Denmark, Kongens Lyngby, DK2800, Denmark. Electronic address:
Tumor markers play a significant role in early cancer diagnosis, evaluation of the extent of the disease, and monitoring of therapy response. In this study, we described the Pickering emulsion polymerization method to synthesize uniform magnetic/fluorescent microspheres. A Pickering-structure composed of a lot silica nanoparticle closely covered onto the quantum dot-encoded magnetic microbeads is designed and synthesized.
View Article and Find Full Text PDFStrand Displacement Amplification Reaction on Quantum Dot-Encoded Silica Bead for Visual Detection of Multiplex MicroRNAs.
Anal Chem
March 2018
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering , Southeast University, Nanjing 210096 , China.
The combination of microbead array, isothermal amplification, and molecular signaling enables the continuous development of next-generation molecular diagnostic techniques. Herein we reported the implementation of nicking endonuclease-assisted strand displacement amplification reaction on quantum dots-encoded microbead (Qbead), and demonstrated its feasibility for multiplexed miRNA assay in real sample. The Qbead featured with well-defined core-shell superstructure with dual-colored quantum dots loaded in silica core and shell, respectively, exhibiting remarkably high optical encoding stability.
View Article and Find Full Text PDFQuantum-dot-encoded silica nanospheres for nucleic acid hybridization.
Small
October 2010
Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Str. 6, 44227 Dortmund, Germany.
Preparation of silica encapsulated quantum dot encoded beads for multiplex assay and its properties.
Anal Biochem
April 2006
Key Laboratory of Biomedical Photonics of Ministry of Education-Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Hubei, People's Republic of China.
Novel -COOH modified polystyrene beads were prepared by sulfonation grafting, and the surface area and pore volume are greatly improved in comparison with the swelling-treated beads. The optimization coating time is 4 h, and the corresponding -COOH content is approximately 2.1 mmol/g.
View Article and Find Full Text PDFQuantum dot-encoded beads.
Methods Mol Biol
July 2005
Department of Biomedical Engineering, Emory University, Atlanta, GA, USA.
Multicolor optical coding for biological assays has been achieved by embedding semiconductor quantum dots into mesoporous and macroporous beads at precisely controlled ratios. Owing to their novel optical properties such as size-tunable emission and simultaneous excitation, quantum dots are ideal fluorophores for wavelength-and-intensity multiplexing. Kinetics study reveals that quantum dot doping of porous silica and polystyrene beads can be completed from seconds to minutes.
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