Mechanical stimuli drive many physiological processes, including touch and pain sensation, hearing, and blood pressure regulation. Mechanically activated (MA) cation channel activities have been recorded in many cells, but the responsible molecules have not been identified. We characterized a rapidly adapting MA current in a mouse neuroblastoma cell line. Expression profiling and RNA interference knockdown of candidate genes identified Piezo1 (Fam38A) to be required for MA currents in these cells. Piezo1 and related Piezo2 (Fam38B) are vertebrate multipass transmembrane proteins with homologs in invertebrates, plants, and protozoa. Overexpression of mouse Piezo1 or Piezo2 induced two kinetically distinct MA currents. Piezos are expressed in several tissues, and knockdown of Piezo2 in dorsal root ganglia neurons specifically reduced rapidly adapting MA currents. We propose that Piezos are components of MA cation channels.
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| http://dx.doi.org/10.1126/science.1193270 | DOI Listing |
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Article Synopsis
- Renin-expressing juxtaglomerular (JG) cells have a mechanism that senses pressure and regulates renin release based on changes in blood flow.
- The study investigates whether Piezo2 receptors, known for their role in detecting touch, play a role in controlling renin synthesis and release in JG cells.
- Results show that Piezo2 channels are not necessary for renin release or synthesis in JG cells under normal or stressed conditions, suggesting that other mechanisms need to be identified.
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