BDNF (brain-derived neurotrophic factor) and its receptor TrkB (tropomyosin receptor kinase B) play important roles in the progression of cancer, including transitional cell carcinoma (TCC) cells reported in our previous investigation. In this study, we used a specific TrkB antibody (Ab) to evaluate its effects on survival, proliferation and migration/invasion in three TCC cell lines (BFTC905, T24 and TSGH8301) in vitro. The TrkB Ab at 1 and 3 microg/ml, but not the TrkA or TrkC Abs, significantly elicited cytotoxicity in TCC cells. The TrkB Ab at 3 microg/ml also induced apoptosis of TCC cells, which may result from up-regulation of phospho-p38 plus down-regulation of survivin and securin expression. The TrkB Ab at 0.5 microg/ml, which did not show cytotoxicity, suppressed migration of TCC cells and invasion of BFTC905 cells, possibly mediated through increased E-cadherin, decreased BDNF-stimulated phospho-PLCgamma1 and reduced MMP-9 activity. These results indicate that TrkB blockade may be a new strategy for TCC therapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3892/ijo_00000745 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Remotely Sequential Activation of Biofunctional MXenes for Spatiotemporally Controlled Photothermal Cancer Therapy Integrated with Multimodal Imaging.
Small
December 2024
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Article Synopsis
- The study introduces TCC@M, a nanoprobe that combines CRISPR-Cas9 gene editing and photothermal therapy to treat cancer more effectively by targeting specific cells at the right time.
- TCC@M is activated by near-infrared light, allowing for controlled release of the Cas9 protein, which specifically alters a gene linked to cancer cell survival, making the cells more susceptible to increased temperatures.
- The research demonstrates that this innovative method not only improves cancer treatment precision but also allows for real-time monitoring through various imaging techniques.
The immune mechanism of the mTOR/ACC1/CPT1A fatty acid oxidation signaling pathway in Hashimoto's thyroiditis.
J Endocrinol Invest
December 2024
Department of Endocrinology and Metabolism, The Second Hospital of Dalian Medical University, Dalian, 116027, People's Republic of China.
Background: Hashimoto's thyroiditis (HT) is the most common autoimmune thyroid disease (AITD), which is distinguished by high thyroid peroxidase antibody (TPOAb) or thyroglobulin antibody (TgAb). The differentiation of CD4T cell subsets in patients with HT is imbalanced, with Treg cells decreased and Th17 cells abnormally activated. Fatty acid oxidation supports the differentiation of Th17 cells and induces inflammation, but the specific mechanism is still unknown.
View Article and Find Full Text PDFCryptotanshinone Suppresses the STAT3/BCL-2 Pathway to Provoke Human Bladder Urothelial Carcinoma Cell Death.
Environ Toxicol
November 2024
Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.
Article Synopsis
- * Cryptotanshinone (CTS), derived from the medicinal herb Danshen, has shown cytotoxic effects on bladder cancer cells while not harming normal cells, primarily through inducing apoptosis.
- * CTS works by inhibiting the JAK2/STAT3/BCL-2 signaling pathway, suggesting its potential as a therapeutic agent for treating bladder cancer.
[Comparison of triclosan and triclocarban in triggering immunotoxicity in larval zebrafish].
Sheng Wu Gong Cheng Xue Bao
October 2024
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, Jiangsu, China.
Article Synopsis
- Triclosan (TCS) and triclocarban (TCC) are effective sterilizers but raise health concerns due to their environmental toxicity, especially in the context of immunotoxic effects.
- A study using zebrafish revealed that both chemicals resulted in low hatching rates and high mortality, with TCC causing more severe developmental issues than TCS.
- TCS increased the proliferation of innate immune cells but reduced mature T cells, while TCC inhibited both types of immune cell differentiation; both pollutants altered pro-inflammatory cytokine levels correlating with toxicity.
Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos.
Theriogenology
January 2025
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye.
The aim of this study was to investigate the effect of putrescine, anti-apoptotic, antioxidant, and a cell proliferation stimulant, on embryo development and quality by supplementing it to in vitro culture medium. In this study, oocytes were obtained from the ovaries of Holstein cattle. Following maturation and fertilization, the presumptive zygotes were randomly assigned to two groups.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!