AI Article Synopsis
- The study demonstrates that human peripheral blood cells can be effectively cultured in commercial serum-free mediums for up to 7 days.
- The research found that higher protein content in serum-free mediums resulted in better cell viability compared to lower protein or serum-containing mediums.
- T cells can be successfully stimulated in serum-free mediums using phytohaemagglutinin, but B lymphocytes showed no IgG secretion when stimulated in the same conditions.
Article Abstract
The cultivation and stimulation of human peripheral blood cells by different mitogens were studied using a serum-free culture technique. It shows the possibility to culture human PBL in a commercially available serum-free medium over a period of at least 7 days. In a serum-free medium with high protein content the viability of the cells was higher than in one with low protein content or in a serum containing medium. It is possible to stimulate human T cells by phytohaemagglutinin in a serum-free medium. After measurement of [3H]thymidine incorporation, a significant difference between control and test cultures was found. There is also a difference between serum-free medium with high and low protein content. Human peripheral B lymphocytes stimulated by pokeweed mitogen did not show any measurable IgG-secretion if they were cultured in serum-free medium.
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