Soluble saccharides are very important metabolites of the life cycle and synthesis of structural polysaccharide components (cellulose, hemicellulose, pectin, etc.) of cell walls. A new method for droplet sampling of saps from tissues of organisms and manipulation routines in capillaries for extraction, derivation, and partitioning were developed for picogram-scale quantitative determination with gas chromatography-mass spectrometry (GC-MS). Five to ten microliters of sap was sampled with a glass capillary containing ribitol (internal standard). Subsequently, the analytes were acetylated with acetic anhydride and catalyzed by 1-methylimidazole. Finally, the soluble saccharides were qualitatively detected with GC-MS SIM (selective ion monitoring) mode. The linear ranges of the method were up to 1×10(-6) mol/L and the theoretically lowest limits of detection (LOD, s/n≥3) were up to 1×10(-9) mol/L. The method is suitable and applicable to analysis of soluble monosaccharides in fresh tissues and other aqueous samples in wide fields of agriculture, food science, biological sciences, and even medical studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf102053h | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Correlation of vitamin D with glucose-lipid metabolism and nutritional status in patients with newly diagnosed type 2 diabetes mellitus.
Pak J Pharm Sci
January 2025
School of Pharmacy, Shaoyang University, Shaoyang, Hunan, China.
Type 2 diabetes mellitus (T2DM) is one of the most common chronic diseases worldwide, with no cure at present. Vitamin D (VD) is a fat-soluble vitamin, which has been recognized as one of the major influencing factors of T2DM. However, the specific relationship between T2DM and VD remains elusive.
View Article and Find Full Text PDFImproving quality of strawberry by novel essential oil nanoemulsions of Echinophora platyloba combined with Aloe vera gel and gum arabic.
Sci Rep
January 2025
Department of Materials Science and Engineering, Faculty of Engineering, Arak University, Arak, 38156-8-8349, Iran.
Strawberry fruits are highly perishable and have a limited shelf life. Therefore, effective methods such as essential oils (EOs) and edible coatings are required to mitigate spoilage and maintain fruit quality during storage. In the current study, Echinophora platyloba EO was extracted and subsequently formulated into a nanoemulsion.
View Article and Find Full Text PDFInsights into potential flavor-active peptides and taste-related compounds in Longjing teas: A comparative study of 'Longjing 43' and 'Qunti' cultivars.
Food Chem
January 2025
Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. Electronic address:
Article Synopsis
- Longjing tea is notable for its rich umami flavor, but the complex chemical interactions that contribute to this taste are not fully understood.
- The study analyzed taste components in two types of Longjing tea: 'Longjing 43' (LJ43) and 'Qunti' (QT), discovering 865 water-soluble peptides in QT and 497 in LJ43, with 44 identified as contributing to umami flavor.
- Findings showed that LJ43 had higher levels of theanine and glutamine than QT, while both varieties contained similar levels of flavan-3-ols, indicating that different chemical compounds and their interactions play a critical role in defining the flavor profile of Longjing tea.
Evaluation of the Anti-Amyloid and Anti-Inflammatory Properties of a Novel Vanadium(IV)-Curcumin Complex in Lipopolysaccharides-Stimulated Primary Rat Neuron-Microglia Mixed Cultures.
Int J Mol Sci
December 2024
Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Lipopolysaccharides (LPS) are bacterial mediators of neuroinflammation that have been detected in close association with pathological protein aggregations of Alzheimer's disease. LPS induce the release of cytokines by microglia and mediate the upregulation of inducible nitric oxide synthase (iNOS)-a mechanism also associated with amyloidosis. Curcumin is a recognized natural medicine but has extremely low bioavailability.
View Article and Find Full Text PDFMustard Meal Extract as an Alternative to Zinc Oxide for Protecting the Intestinal Barrier Against -Lipopolysaccharide Damage.
Int J Mol Sci
December 2024
Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, 077015 Ilfov, Romania.
The present study aimed to investigate the ability of an aqueous extract derived from mustard seed meal to counteract the effects of endotoxin lipopolysaccharide (LPS) on the intestinal epithelium. Caco-2 cells were cultured together with HT29-MTX and used as a cellular model to analyze critical intestinal parameters, such as renewal, integrity, innate immunity, and signaling pathway. Byproducts of mustard seed oil extraction are rich in soluble polysaccharides, proteins, allyl isothiocyanates, and phenolic acids, which are known as powerful antioxidants with antimicrobial and antifungal properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!