AI Article Synopsis
- A microfluidic system was developed for liquid flow in a specimen chamber designed for scanning transmission electron microscopy (STEM) imaging.
- This system features ultrathin silicon nitride windows and microchips, allowing for real-time imaging of samples while isolated from the electron microscope's vacuum.
- The system can rapidly exchange liquids, opening possibilities for studying how various specimens, like cells, respond to different stimuli.
Article Abstract
We present a microfluidic system that maintains liquid flow in a specimen chamber for scanning transmission electron microscope (STEM) imaging. The specimen chamber consists of two ultrathin silicon nitride windows supported by silicon microchips. They are placed in a specimen holder that seals the sample from the vacuum in the electron microscope and incorporates tubing to and from the sample connected to a syringe pump outside the microscope. Using results obtained from fluorescence microscopy of microspheres flowing through the system, an equation to characterize the liquid flow through the system was calibrated. Gold nanoparticles of diameters of 30 and 100 nm moving in liquid were imaged with a 200 kV STEM. It was concluded that despite strong influences from Brownian motion, and sensitivity to small changes in the depth of the bypass channel, the electron microscopy flow data matched the calculated flow speed within an order of magnitude. The system allows for rapid (within a minute) liquid exchange, which can potentially be used, for example, to investigate the response of specimens, e.g., eukaryotic or bacterial cells, to certain stimuli.
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| http://dx.doi.org/10.1017/S1431927610093669 | DOI Listing |
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