Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/adma.201002234 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes.
mBio
December 2024
Department of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, Michigan, USA.
Article Synopsis
- The nickel-pincer nucleotide (NPN) cofactor is essential for certain enzymes known as racemases and epimerases, and is synthesized by specific enzymes (LarB, LarC, and LarE) before being installed into LarA homologs.
- Cloning these genes into the Duet expression system enables the production of active LarA homologs and allows functional testing of homologs from other microorganisms.
- Circular dichroism spectroscopy is introduced as a new method to monitor enzyme activities efficiently by tracking the conversion of specific substrates without needing extra reagents.
Walnut-derived peptides cross the blood-brain barrier and ameliorate Aβ-induced hypersynchronous neural network activity.
Food Res Int
December 2024
College of Food Science and Engineering, Jilin Agricultural University, ChangChun, Jilin 130118, PR China. Electronic address:
Walnut peptides exhibit promising neuroprotective effects; however, they must be absorbed in their intact form through the gastrointestinal tract into the bloodstream and brain. In this study, the effects of the walnut peptide TWLPLPR (TW-7) were evaluated in mice, including its absorption and distribution ability to cross the blood-brain barrier, and inhibitory effects on hyperactivity of primary hippocampal neurons. TW-7 was stable in plasma, and the peptide retention rate was 88.
View Article and Find Full Text PDFTopological DNA blends exhibit resonant deformation fields and strain propagation dynamics tuned by steric constraints.
Acta Biomater
December 2024
Department of Physics and Biophysics, University of San Diego, 5998 Alcala Park, San Diego, CA 92110, United States. Electronic address:
Understanding how polymers deform in response to local stresses and strains, and how strains propagate from a local disturbance, are grand challenges in wide-ranging fields from materials manufacturing to cell mechanics. These dynamics are particularly complex for blends of polymers of distinct topologies, for which several different species-dependent mechanisms may contribute. Here, we use OpTiDDM (Optical Tweezers integrating Differential Dynamic Microscopy) to elucidate deformation fields and propagation dynamics of binary blends of linear, ring and supercoiled DNA of varying sizes.
View Article and Find Full Text PDFDiscovering genotype-phenotype relationships with machine learning and the Visual Physiology Opsin Database (VPOD).
Gigascience
January 2024
Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, California 93106, USA.
Background: Predicting phenotypes from genetic variation is foundational for fields as diverse as bioengineering and global change biology, highlighting the importance of efficient methods to predict gene functions. Linking genetic changes to phenotypic changes has been a goal of decades of experimental work, especially for some model gene families, including light-sensitive opsin proteins. Opsins can be expressed in vitro to measure light absorption parameters, including λmax-the wavelength of maximum absorbance-which strongly affects organismal phenotypes like color vision.
View Article and Find Full Text PDFHistopathologic and ultrastructural findings in oak ( spp.) toxicity on 2 beef cattle farms in Colorado.
J Vet Diagn Invest
October 2024
Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biological Sciences, Colorado State University, Fort Collins, CO, USA.
Article Synopsis
- - Natural oak toxicity occurs when ruminants like cattle consume parts of oak trees, leading to severe health issues due to the breakdown of plant tannins by gut microbes and enzymes.
- - Symptoms of acute oak toxicity resemble those of renal disease, specifically acute tubular injury, which can be fatal.
- - A study of three beef calves in Colorado revealed significant kidney damage and unique microscopic features of oak-induced toxicity, highlighting previously undocumented effects on renal tissues and blood vessels.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!