AI Article Synopsis
- Alkaliphilic Bacillus species, which thrive in high pH environments, rely on unknown biochemical mechanisms to acquire iron and show sensitivity to iron-chelating agents.
- These bacteria produce unique iron-chelating molecules called siderophores that contain specific chemical structures (catechol and hydroxamate).
- The production of these siderophores is influenced by manganese salts and is inhibited by the presence of iron chloride, indicating a complex regulatory mechanism.
Article Abstract
The biochemical and molecular mechanisms used by alkaliphilic bacteria to acquire iron are unknown. We demonstrate that alkaliphilic (pH > 9) Bacillus species are sensitive to artificial iron (Fe³+) chelators and produce iron-chelating molecules. These alkaliphilic siderophores contain catechol and hydroxamate moieties, and their synthesis is stimulated by manganese(II) salts and suppressed by FeCl₃ addition. Purification and mass spectrometric characterization of the siderophore produced by Caldalkalibacillus thermarum failed to identify any matches to previously observed fragmentation spectra of known siderophores, suggesting a novel structure.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953014 | PMC |
| http://dx.doi.org/10.1128/AEM.01393-10 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Selection of alkaliphilic Bacillus pectate lyases based on reactivity and pH-dependent stability in simulated environment for industrial applications.
Carbohydr Res
March 2025
Quantitative Biology Lab, Department of Integrative Biology, School of Bio Sciences and Technology, Vellore Institute of Technology (VIT Deemed to Be University), Vellore, Tamil Nadu, India. Electronic address:
Pectate lyases, known for their alkaliphilic nature, are ideal for industrial applications that require specific pH conditions, particularly in industries such as textiles and pulp extraction. These enzymes, primarily from the polysaccharide lyase family 1 (PL1) of different microbial sources, play a vital role in polysaccharide degradation. Given the potent pectinolytic activity of Bacillus pectate lyases, targeting these enzymes is crucial for identifying the most effective candidates.
View Article and Find Full Text PDFEditorial expression of concern:Characterization and high-efciency secreted expression in bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic bacillus clausii strain S10.
Microb Cell Fact
November 2024
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Halophilic Phosphate-Solubilizing Microbes (Priestia megaterium and Bacillus velezensis) Isolated from Arabian Sea Seamount Sediments for Plant Growth Promotion.
Curr Microbiol
October 2024
Marine Biotechnology Division, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai, 600100, India.
Arabian Sea is a highly productive Ocean owing to deep upwelling with reports on phosphorus cycling in ocean sediments. In this study, microbes from sea mounts of the Arabian Sea at varying depths (400 m, 900 m) were screened to isolate and characterize phosphate-solubilizing bacteria (PSB) with plant growth-promoting properties. Out of the seven morphologically different PSBs, two bacterial strains with maximum phosphate solubilization index were identified as Priestia megaterium (H1) and Bacillus velezensis (H2) based on biochemical and molecular characteristics.
View Article and Find Full Text PDFImproved methods for genetic manipulation of the alkaliphile .
Front Microbiol
September 2024
Howard Hughes Medical Institute, Yale University, New Haven, CT, United States.
An improved approach was developed for the genetic manipulation of the Gram-positive extremophile (formerly called ). We describe an allelic replacement method originally developed for that allows the deletion, mutation, or insertion of genes without leaving markers or other genetic scars. In addition, a protocol for rapid plasmid methylation and transformation is presented.
View Article and Find Full Text PDFMetabolic and Morphological Aspects of Adaptation of Alkaliphilic 5-DB and Alkali-Tolerant ATCC 6633 to Changes in pH and Mineralization.
Int J Microbiol
July 2024
Laboratory of Molecular Biotechnology Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences, Golev Str., 13, Perm 614081, Russia.
The goal of the study is to evaluate metabolic and morphological changes of the facultative alkaliphile 5-DB and the weakly alkali-resistant ATCC 6633 in a wide pH range and at different NaCl concentrations. The alkaliphile 5-DB is shown to have a broader general resistance to adverse factors (wide pH range, 50 g/L NaCl) than a weakly alkali-tolerant strain of the same genus. This alkaliphile is also shown to have a significantly greater resistance not only to high pH but also to low pH in comparison with ATCC 6633.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!