We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2010.08.012 | DOI Listing |
Publication Analysis
Top Keywords
staphylococcus aureus
8
membrane potential
8
rapid microtiter
4
microtiter plate
4
plate assay
4
assay measuring
4
compounds
4
measuring compounds
4
compounds staphylococcus
4
aureus membrane
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!