A general strategy to enzymatically label acyl carrier proteins (ACPs) of polyketide synthases has been developed. Incorporation of a chloromethyl ketone or vinyl ketone moiety into polyketide chain elongation intermediate mimics allows for the synthesis of CoA adducts. These CoA adducts undergo enzymatic reaction with Sfp, a phosphopantetheinyl transferase, to afford labeled CurB carrier proteins.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2992964 | PMC |
| http://dx.doi.org/10.1016/j.bmcl.2010.05.089 | DOI Listing |
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