In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio.

J Fish Biol

Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg-Munich, Germany.

Published: February 2009

The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.

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Source
http://dx.doi.org/10.1111/j.1095-8649.2008.02075.xDOI Listing

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