AI Article Synopsis
- The study investigates the therapeutic effects of transplanting bone marrow-derived mesenchymal stromal cells (MSC) into a mouse model of Duchenne muscular dystrophy.
- Behavioral and locomotor tests showed significant improvement in mobility and increased expression of dystrophin and utrophin in treated mice over a period of 15 weeks post-transplant.
- The findings suggest that MSC transplantation could be a valuable treatment approach for muscular dystrophy, although the median lifespan of treated mice remained shorter than that of healthy mice.
Article Abstract
Background Aims: We explored the potential therapeutic value of transplanting bone marrow (BM)-derived mesenchymal stromal cells (MSC) into utrophin/dystrophin-deficient double knock-out (dko) mice, a murine model of Duchenne muscular dystrophy.
Methods: MSC from male rats were isolated and transplanted into female dko mice via the caudal vein. Behavior and locomotor function were later evaluated, along with the expression of dystrophin and utrophin in the sarcolemma of myofiber tissues. The presence of grafted cells was confirmed via polymerase chain reaction for the sex-determining region of the Y-chromosome.
Results: Locomotor activity improved significantly (P < 0.05) from 5 to 15 weeks after cell transplantation, as measured by traction, rotating rod and running wheel tests. We also found that the expression of dystrophin and utrophin increased significantly (P < 0.05) and progressively in the sarcolemma from 5 to 15 weeks after transplantation. The median lifespan of mice in the normal group (74.1 weeks) was significantly (P < 0.001) higher than those in the control (22.0 weeks) and transplantation (35.0 weeks) groups, and the median lifespan of mice in the transplantation group was significantly (P < 0.001) higher than that in the control group.
Conclusions: Results of this study demonstrate that BM MSC have potential value in xenogeneic transplantation therapy for muscular dystrophy.
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| http://dx.doi.org/10.3109/14653249.2010.510502 | DOI Listing |
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