AI Article Synopsis
- - A new method is introduced for measuring glycogen levels in tissues while keeping their structure intact, using iodine for staining.
- - This technique ensures that the results are not affected by other polysaccharides present, as they do not react with iodine but instead break down into reducing sugars during hydrolysis.
- - The quantification of glycogen is then derived from a calibration curve created for this staining process.
Article Abstract
A simple specific method for the detection of glycogen concentration in various tissues without disintegration of its structure is described. The method includes staining of glycogen molecules with iodine. The results of determination do not depend on the presence of polysaccharides because they are not stained with iodine but release reducing sugars during hydrolysis. Glycogen content is calculated from the calibration curve.
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