Analyzing shoot apical meristem development.

Methods Mol Biol

Plant Gene Expression Center, U.S. Department of Agriculture-ARS/UC Berkeley & Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.

Published: December 2010

AI Article Synopsis

  • The shoot apical meristem (SAM) of Arabidopsis thaliana is a crucial area that contains pluripotent stem cells responsible for the continuous generation of new cells needed for organ development.
  • During embryogenesis, the SAM becomes layered and can be classified into different zones based on physical and molecular characteristics.
  • Changes in the SAM's structure or function can significantly impact the plant's shape and reproductive capabilities, with specific defects assessable through advanced microscopy and histology methods.

Article Abstract

The shoot apical meristem of Arabidopsis thaliana contains a reservoir of pluripotent stem cells that functions as a continuous source of new cells for organ formation during development. The SAM forms during embryogenesis, when it becomes stratified into specific cell layers and zones that can be delineated based on morphological and molecular criteria. The primary SAM produces all the aerial structures of the adult plant, and alterations in SAM organization or function can have profound effects on vegetative and reproductive plant morphology. Such SAM-specific defects can be identified, evaluated, and quantified using specialized microscopic and histological techniques.

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http://dx.doi.org/10.1007/978-1-60761-765-5_8DOI Listing

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