Background: Lung cancer is the most dangerous threating tumor to human's health and life. Metastasis is not only the malignant characteristics of lung cancer, but also the chief cause of failure to cure the disease and of high mortality. Ginsenoside Rg3 has been proved to have obvious effect against tumor. The molecular mechanism of Rg3 against lung cancer cell line will be investigated by using two-dimensional gel electrophoresis in this paper.
Methods: The IC50 of Rg3 against human high-metastatic large cell lung cancer cell line 95D was determined by MTT. Then 95D cells were treated with Rg3 at the concentration of 0.1*IC50 for 72 h. The total proteins of 95D cell line treated with Rg3 and not treated were separated and protein profiles were obtained by using immobilized PH gradient (IPG)-based two-dimensional gel electrophoresis. The differential expression proteins of 95D cell line treated with Rg3 and not treated were analyzed using image analysis software.15 of differentially expressed proteins were further identified using MALDI-TOF MS/MS analysis and LC-MS/MS analysis. Protein identification was performed by searching the protein database.
Results: The IC50 of Rg3 against 95D cell line was 100 mug/mL. There were 27 differently expressed protein spots through analysis by Image Master Microsoft.15 proteins were identified using mass spectrometry. Chloride intracellular channel protein 1, Ubiquitin-Conjugating Enzyme E2-25 Kda only expressed in control; 14-3-3 protein teta, SKI-interacting protein only expressed in 95D cell line treated by Rg3; Annexin A2, profilin 2 isoform b were downregulated in 95D cell line treated by Rg3; 14-3-3 protein zeta, Eukaryotic translation initiation factor 4H were upregulated in 95D cell line treated by Rg3.
Conclusions: A significantly different expression of proteins were found in 95D cell line treated with Rg3 and those not treated. Most of identified proteins have been reported to be associated with tumor metastasis. The identified proteins will provide the basis for searching potential biomarkers and the molecular mechanism of Rg3 against the metastasis of lung cancer cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3779/j.issn.1009-3419.2008.03.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository.
Poult Sci
November 2024
The Roslin Institute, University of Edinburgh, Easter Bush Campus, Edinburgh, EH25 9RG, UK. Electronic address:
Article Synopsis
- A new method has been developed to directly introduce frozen embryonic gonadal germ cells (GGC) into sterile chicken hosts to help preserve chicken breeds and their genetic materials.
- The technique is particularly efficient using male gonads, but obtaining enough viable female gonads for breeding is challenging due to limited egg production in certain chicken breeds.
- A simplified culture method allows for high-purity GGC to be recovered from frozen 9.5-day-old embryonic gonads, supporting the biobanking of indigenous chicken breeds and potentially other bird species.
Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice.
bioRxiv
September 2024
Tri-Institutional Training Program in Laboratory Animal Medicine and Science, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, NY.
(Cm) has reemerged as a moderately prevalent infectious agent in research mouse colonies. Despite its' experimental use, few studies evaluate Cm's effects on immunocompetent mice following its natural route of infection. A Cm field isolate was administered (orogastric gavage) to 8-week-old female BALB/cJ (C) mice.
View Article and Find Full Text PDFSe-methylselenocysteine inhibits the progression of non-small cell lung cancer via ROS-mediated NF-κB signaling pathway.
Exp Cell Res
July 2024
Department of Pulmonary and Critical Care Medicine, Fujian Medical University Union Hospital, Fuzhou, Fujian, China. Electronic address:
Article Synopsis
- Se-methylselenocysteine (MSC) shows promise for preventing cancer, particularly in a type of lung cancer called non-small cell lung cancer (NSCLC).
- In tests, MSC slowed down cancer cell growth, reduced their ability to move and invade, and made them die more often.
- The research also found that MSC worked by affecting certain pathways in the cells and showed it could shrink tumors in mice, making it a possible treatment for NSCLC.
Ginsenoside Rb1 Inhibits the Proliferation of Lung Cancer Cells by Inducing the Mitochondrial-mediated Apoptosis Pathway.
Anticancer Agents Med Chem
July 2024
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130117, China.
Background: Lung cancer is one of the more common malignant tumors posing a great threat to human life, and it is very urgent to find safe and effective therapeutic drugs. The antitumor effect of ginsenosides has been reported to be a treatment with a strong effect and a high safety profile.
Objective: This paper aimed to investigate the inhibitory effect of ginsenoside Rb1 on 95D and NCI-H460 lung cancer cells and its pathway to promote apoptosis.
Anti-collagenase, Anti-elastase, Anti-urease, and Anti-cancer Potentials of Isokaempferide as Natural Compound: In vitro and in silico Study.
J Oleo Sci
February 2024
Department of Biology, College of Science, King Khalid University.
One of the main goals of medicinal chemistry in recent years has been the development of new enzyme inhibitors and anti-cancer medicines. The isokaempferide' ability to inhibit the enzymes urease, elastase, and collagenase were also studied. The results showed that isokaempferide was the most effective compound against the assigned enzymes, with IC values of 23.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!