Objectives: To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput.
Design And Methods: Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coefficient of variation (CV), method comparison with a radioenzymatic assay (REA) and liquid chromatography tandem mass spectrometry assay (LC-MS/MS) were performed. The method was tested for interferences and for long-term performance.
Results: The method LOD and LOQ were 0.87 and 1.36 μmol/L for free carnitine and 1.79 and 2.54 μmol/L for total carnitine, respectively. No interferences were detected. Long-term measurement CVs for both free and total carnitine were <4%.
Conclusions: The method shows acceptable performance characteristics with improved speed and low level accuracy compared to existing REA and MS/MS methods.
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