Genotyping of Legionella strains is important for the epidemiologic survey of Legionnaires' disease infections. In this study, we investigated the potential of repetitive element-polymerase chain reaction (rep-PCR) for differentiating various isolates of Legionella spp. We used 38 Legionella pneumophila isolates (collected in clinics all over Japan between 1980 and 2007), 19 environmental Legionella anisa isolates (collected in Okinawa, Nara, Osaka, and Hyogo prefecture between 1987 and 2007), and 2 Legionella-type strains. We extracted bacterial genomic DNA and applied it to rep-PCR. PCR products were then converted into bands by agarose gel electrophoresis. The L. pneumophila serogroup (SG) 1 displayed very diverse patterns. Different bands were produced for each species of Legionella, and each species was clearly distinct. Phylogenetic analysis displayed 1 cluster of L. anisa isolates, while other Legionella spp. were present at discrete levels. Our findings show that rep-PCR is an effective, rapid, and simple technique for differentiation of L. pneumophila strains as well as Legionella spp.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.diagmicrobio.2010.04.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Vertical stratification of the water microbiome in an electric water heater tank: implications for premise plumbing opportunistic pathogens.
J Water Health
December 2024
US Environmental Protection Agency, Office of Research and Development, Cincinnati, OH 45268, USA.
Hot water systems are the most frequent environment associated with the prevalence and growth of opportunistic premise plumbing pathogens (OPPPs). Previous studies identified water heaters as a source of waterborne diseases and concluded that design variables may contribute to their prevalence. A multifaceted approach was used to investigate the vertical stratification of the microbiome and selected OPPPs in an electric water heater tank connected to a home plumbing system simulator.
View Article and Find Full Text PDFSynergistic effects of ozonation pretreatment and trace phosphate on water quality health risk and microbial stability in simulated drinking water distribution systems.
J Hazard Mater
December 2024
Institute of Environmental Research at Greater Bay, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China.
The proliferation and chlorine resistance of pathogenic bacteria in drinking water distribution systems (DWDSs) pose a serious threat to human health. In this study, the synergistic effects of ozonation pretreatment and trace phosphate on water quality health risk and microbial stability were investigated in the small-scale DWDSs simulated by biofilms annular reactors with cast iron coupons. The results indicated that ozonation of drinking water containing trace phosphate was equivalent to increasing microbial carbon and phosphorus sources, further leading to the rapid proliferation of opportunistic pathogens (OPs) in subsequent DWDSs.
View Article and Find Full Text PDFMeningitis due to Gemella sp. in a patient with severe ENT conditions: case report and review of the literature.
Ann Clin Microbiol Antimicrob
December 2024
Laboratoire de Microbiologie, Centre Hospitalier de Valenciennes, Valenciennes, F-59300, France.
In June 2022, a 73-year-old man with a history of laryngeal and esophageal carcinoma was admitted to the emergency unit with sudden fever, confusion, and general condition deterioration. Initial assessments showed a fever of 38.5 °C, elevated C-reactive protein (CRP) at 209 mg/L, and a neutrophil count of 10.
View Article and Find Full Text PDFDetection of spp. in influent wastewater in Kobe City, Japan.
J Water Health
November 2024
Department of Infectious Diseases, Kobe Institute of Health, Kobe, Japan E-mail:
Article Synopsis
- The text discusses the investigation of an important waterborne pathogen associated with Legionnaires' disease (LD) found in wastewater treatment plants (WWTPs) in Kobe, Japan, from April 2023 to March 2024.
- Researchers discovered that the pathogen was present in varying concentrations across all seasons, with one specific species being the most common.
- The study emphasizes the potential risk of LD from influent wastewater in Japan, particularly in the context of frequent natural disasters, highlighting the need for ongoing monitoring and research.
Analysis of water and aerosol samples of tunnel car washes operated with recycled water for Legionella with culture, qPCR and viability-qPCR.
Sci Total Environ
December 2024
Department of Occupational and Environmental Health, Epidemiology, Bavarian Health and Food Safety Authority, Munich, Germany; Institute and Clinic for Occupational, Social and Environmental Medicine, University Hospital, LMU Munich, Munich, Germany.
Due to the generation of large quantities of aerosol and the recycling of water, tunnel car washes are discussed as potential sources of legionellosis. Additionally, occupational health and safety aspects are important for tunnel car washes as they are often workplaces. A total of 17 different tunnel car washes were investigated for the presence of Legionella.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!