AI Article Synopsis
- Osteopontin (OPN) is a glycophosphoprotein that plays a role in promoting the migration of immune cells, such as neutrophils and macrophages, which may contribute to cancer progression.
- In cancer types like glioblastoma, high levels of OPN are found, and its expression is significantly heightened, especially in invasive tumors compared to normal tissues.
- The study demonstrated that OPN not only facilitates the movement of cancer cells but also attracts immune cells to tumor sites, suggesting a dual role in cancer dynamics.
Article Abstract
Osteopontin (OPN) is a glycophosphoprotein with multiple intracellular and extracellular functions. In vitro, OPN enhances migration of mouse neutrophils and macrophages. In cancer, extracellular OPN facilitates migration of cancer cells via its RGD sequence. The present study was designed to investigate whether osteopontin is responsible for neutrophil and macrophage infiltration in human cancer and in particular in glioblastoma. We found that in vitro mouse neutrophil migration was RGD-dependent. In silico, we found that the OPN gene was one of the 5% most highly expressed genes in 20 out of 35 cancer microarray data sets in comparison with normal tissue in at least 30% of cancer patients. In some types of cancer, such as ovarian cancer, lung cancer and melanoma, the OPN gene was one of those with the highest expression levels in at least 90% of cancer patients. In glioblastoma, the most invasive type of brain tumours/glioma, but not in lower grades of glioma it was one of the 5% highest expressed genes in 90% of patients. In situ, we found increased protein levels of OPN in human glioblastoma versus normal human brain confirming in silico results. OPN protein expression was co-localized with neutrophils and macrophages. In conclusion, OPN in tumours not only induces migration of cancer cells but also of leucocytes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3015073 | PMC |
| http://dx.doi.org/10.1111/j.1365-2567.2010.03335.x | DOI Listing |
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