A perhydro-26-membered hexaazamacrocycle-based silica (L(1)GlySil) stationary phase for high-performance liquid chromatography (HPLC) was prepared using 3-glycidoxypropyltrimethoxysilane as coupling reagent. The structure of new material was characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. The chromatographic performance and retention mechanism of the new phase were evaluated in reversed-phase (RP) and normal-phase (NP) modes using different solute probes including aromatic compounds, organophosphorus pesticides, carbamate pesticides and phenols. The results showed that L(1)GlySil was a sort of multimode-bonded stationary phase with excellent chromatographic properties. The new phase could provide various action sites for different solutes, such as hydrophobic, hydrogen bonding, pi-pi, dipole-dipole interactions and acid-base equilibrium. The presence of phenyl rings, secondary amino groups and alkyl linkers in the resulting material made it suitable for the separation of above-mentioned analytes by multimode retention mechanisms.
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