Pluripotent human embryonic stem (hES) cells are an important experimental tool for basic and applied research, and a potential source of different tissues for transplantation. However, one important challenge for the clinical use of these cells is the issue of immunocompatibility, which may be dealt with by the establishment of hES cell banks to attend different populations. Here we describe the derivation and characterization of a line of hES cells from the Brazilian population, named BR-1, in commercial defined medium. In contrast to the other hES cell lines established in defined medium, BR-1 maintained a stable normal karyotype as determined by genomic array analysis after 6 months in continuous culture (passage 29). To our knowledge, this is the first reported line of hES cells derived in South America. We have determined its genomic ancestry and compared the HLA-profile of BR-1 and another 22 hES cell lines established elsewhere with those of the Brazilian population, finding they would match only 0.011% of those individuals. Our results highlight the challenges involved in hES cell banking for populations with a high degree of ethnic admixture.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3727/096368910X522261 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Physician-Visit Frequency and Its Impact on Glycemic Control in People With Type 2 Diabetes: Quantifying Care Acceptance Parameters From Retrospective Electronic Health Record Data.
Cureus
December 2024
Obstetrics and Gynecology, Shree Guru Gobind Singh Tricentenary University Medical College, Hospital and Research Institute, Gurugram, IND.
Objective: Type 2 diabetes is a metabolic disorder characterized by insulin resistance and hyperglycemia affecting many individuals worldwide. For effective management, adherence to recommended physician visits is important, along with lifestyle modification and pharmacological interventions. Regular doctor visits can improve adherence and help prevent complications.
View Article and Find Full Text PDFPhysical scaling for predicting shear viscosity and memory effects of lithium-ion battery cathode slurries.
Soft Matter
January 2025
Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
Lithium-ion battery cathodes are manufactured by coating slurries, liquid suspensions that typically include carbon black (CB), active material, and polymer binder. These slurries have a yield stress and complex rheology due to CB's microstructural response to flow. While optimizing the formulation and processing of slurries is critical to manufacturing defect-free and high-performance cathodes, engineering the shear rheology of cathode slurries remains challenging.
View Article and Find Full Text PDFResilience-based interventions in the public sector workplace: a systematic review.
BMC Public Health
January 2025
Erasmus School of Social and Behavioural Sciences, Erasmus University Rotterdam, Burgemeester Oudlaan 50, 3062 PA, Rotterdam, The Netherlands.
Background: Previous studies have advocated the benefits of resilience-based interventions for creating a healthy and sustainable workforce. However, resilience is defined and measured in diverse ways. Therefore, the aim of this systematic review is (1) to identify how resilience is defined within different workplace interventions, translated into intervention content, and measured in these interventions; and (2) to synthesize the effectiveness of these interventions.
View Article and Find Full Text PDFInfluence of matrix stiffness on microstructure evolution and magnetization of magneto-active elastomers.
Soft Matter
January 2025
Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, Dresden, 01069, Germany.
Field-induced microstructure evolution can play an important role in defining the coupled magneto-mechanical response of Magneto-Active Elastomers (MAEs). The behavior of these materials is classically modeled using mechanical, magnetic and coupled magneto-mechanical contributions to their free energy function. If the MAE sample is fully clamped so it cannot deform, the mechanical coupling is reduced to the internal microscopic deformations caused by the particles moving and deforming the elastic medium that surrounds them.
View Article and Find Full Text PDFFirst-Attempt Success in Ultrasound-Guided vs Standard Peripheral Intravenous Catheter Insertion: The EPIC Superiority Randomized Clinical Trial.
JAMA Pediatr
January 2025
Vascular Assessment and Management Service, Department of Anaesthesia and Pain, Queensland Children's Hospital, Children's Health Queensland Hospital and Health Service, Queensland, Australia.
Importance: Pediatric peripheral intravenous catheter (PIVC) insertion can be difficult and time-consuming, frequently requiring multiple insertion attempts and often resulting in increased anxiety, distress, and treatment avoidance among children and their families. Ultrasound-guided PIVC insertion is a superior alternative to standard technique (palpation and visualization) in high-risk patients.
Objective: To compare first-time insertion success of PIVCs inserted with ultrasound guidance compared with standard technique (palpation and visualization) across all risk categories in the general pediatric hospital population.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!