Objective: The purpose of this study is to express partial S gene of Hantavirus Z10.
Methods: The 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T. The SP300 gene was constructed into pPICZaA and sequenced. The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCI.
Results: The recombination Pichia were cultivate, and expressed the SP300 or S300 gene induced in Pichia by methanol.
Conclusion: The nucleocapsid secreted from the Pichia can be detected by ELISA and WesternBlot.
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