The enzymatic cleaning of a rubisco protein stain bound onto Surface Plasmon Resonance (SPR) biosensor chips having a dye-bound upper layer is investigated. This novel method allowed, for the first time, a detailed kinetic study of rubisco cleanability (defined as fraction of adsorbed protein removed from a surface) from dyed surfaces (mimicking fabrics) at different enzyme concentrations. Analysis of kinetic data using an established mathematical model able to decouple enzyme transfer and reaction processes [Onaizi, He, Middelberg, Chem. Eng. Sci. 64 (2008) 3868] revealed a striking effect of dyeing on enzymatic cleaning performance. Specifically, the absolute rate constants for enzyme transfer to and from a dye-bound rubisco stain were significantly higher than reported previously for un-dyed surfaces. These increased transfer rates resulted in higher surface cleanability. Higher enzyme mobility (i.e., higher enzyme adsorption and desorption rates) at the liquid-dye interface was observed, consistent with previous suggestions that enzyme surface mobility is likely correlated with overall enzyme cleaning performance. Our results show that reaction engineering models of enzymatic action at surfaces may provide insight able to guide the design of better stain-resistant surfaces, and may also guide efforts to improve cleaning formulations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcis.2010.07.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The co-production of xylose, fermentable glucose and β-O-4 linkage-rich lignin through efficiently dismantling sugarcane bagasse.
Int J Biol Macromol
December 2024
Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China. Electronic address:
As an "upstream" process in biorefinery, biomass dismantling can dismantle the natural stable structure of lignocellulosic biomass and separate its three major components. To increase the value of the entire biomass by fully utilizing the three main components (cellulose, lignin, and hemicellulose), this study proposes a two-step decomposition system combining formic acid (FA) pretreatment and ethylene glycol-NaOH (EGA) dismantling, aiming to effectively convert sugarcane bagasse into xylose, fermentable glucose, and high-value lignin. In the first step, FA pretreatment removed 79.
View Article and Find Full Text PDFProduction of Novel Energy Gases in Bioprocesses Using Undefined Mixed Cultures.
Adv Biochem Eng Biotechnol
December 2024
Savonia University of Applied Sciences, Kuopio, Finland.
Three phases of matter intermingle in various environments. The phenomena behind these fluctuations provide microbial cultures with beneficial interphase on the borderlines. Correspondingly, a bioreactor broth usually consists of a liquid phase but also contains solid particles, gas bubbles, technical surfaces, and other niches, both on a visible scale and microscopically.
View Article and Find Full Text PDFThe enzymatic and transcriptional adverse effects of hull in-water cleaning discharge on juvenile rockfish (Sebastes schlegeli).
Aquat Toxicol
December 2024
Ecological Risk Research Department, Korea Institute of Ocean Science and Technology, Geoje, 53201, South Korea; Department of Ocean Science, Korea University of Science and Technology, Daejeon, 34113, South Korea. Electronic address:
The hull in-water cleaning (IWC) process creates chemical contaminants, including antifouling paint particle mixtures that are directly discharged into the coastal environment. Recent attention has also been paid to the international regulation of ship hull cleaning discharges in environmental media. However, few studies have investigated the adverse effects or toxic pathways on resident marine species.
View Article and Find Full Text PDFUnveiling the mechanisms of mixed surfactant synergy in passivating lignin-cellulase interactions during lignocellulosic saccharification.
J Colloid Interface Sci
March 2025
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Article Synopsis
- Surfactants can improve the enzymatic breakdown of lignocellulosic biomass, leading to more sugar yield with less enzyme needed, but how they interact with lignin and cellulase remains unclear.
- A combination of non-ionic and cationic surfactants notably reduced the non-productive attachment of lignin to cellulase—by up to 55%—while promoting conditions that favor cellulase activity.
- The effectiveness of these surfactants in diminishing lignin's impact on cellulases is influenced by lignin's properties, suggesting that future research can inform better surfactant or additive designs for more efficient biomass processing.
Bio-mitigation of CO: The co-production of phycocyanin and optically pure (R)-γ-valerolactone with perfect enantiomeric excess.
Bioresour Technol
November 2024
Gwagnju Clean Energy Research Center, Gwangju 61003, Republic of Korea. Electronic address:
To overcome the climate crisis, various greenhouse gas (GHG) mitigation strategies have been developed, and every effort has been made to achieve carbon neutrality. Given that petroleum-based industries and the transportation sector emit enormous amounts of GHGs, the product spectra of biorefineries should be expanded beyond drop-in biofuels to include more value-added products. This study aimed to construct a CO mitigation system.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!