[Identification of sibling sisters using STR and SNP].

Objective: The methods for identification of sibling sisters were explored with detection of genetic markers on autochromosome and X-chromosome.

Methods: Genomic DNA of the sibling sisters were extracted, and 15 STRs on autochromosome and 17 STRs on X-chromosome were genotyped by Sinofiler kit, Mentype Argus X-8 kit and in-house kit of X-STRs, respectively. 11 X-SNPs were genotyped with TaqMan technology.

Results: Full sibling relationship of the test samples were confirmed by calculating full sibling index of STRs in autochromosome, which were also supported by the detection of 1-2 same alleles at each locus on X-chromosome.

Conclusion: In identification of full sibling sister, not only STRs on autochromosome but also polymorphism genetic markers in X-chromosome can be utilized.