With the reversed passive hemagglutination technique it is possible to detect minimal amounts of botulinum type A, B and E toxins (s. Tab. 3). The antisera are used were prepared by foot--pad injection of rabbits with purified toxoids in Freund's complete adjuvant (s. Tab. 1). Antitoxin globulin were prepared from rabbit antisera with (NH4)2SO4 to 50%. Formalinized and tanned human erythrozytes were sensitized with these specific antitoxin globulins. Only slight cross reactions ere encountered between the type A, B, and E antiglobulin sensitized cells and culture filtrates of C. butyricum, C. sporogenes (type A and B antiglobulin only) and C. perfringens type C (s. Tab. 4).
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