The performance of a sulfidogenic bioreactor and the response of bacterial populations to influent alkalinity changes were investigated. The bioreactor reached 40% of sulfate removal efficiency (SRE) with 0 mg l(-1) of alkalinity, and single-stranded conformation polymorphism profiles showed that some members of Bacteroides, Dysgonomonas, Sporobacter, Quinella, and Citrobacter became dominant populations. 16S rRNA gene library analysis indicated that the Actinobacteria group increased from 0% in seed to 23% in sludge. An increase in alkalinity to 1300 mg l(-1) led to a rapid increase of SRE to 65% and changes in the bacterial community. Sequences representing Dysgonomonas, Raoultella, Kluyvera, and Phascolarctobacterium were now found. When alkalinity was deceased to 0 mg l(-1), SRE dropped and the bands representing Raoultella, Kluyvera, and Phascolarctobacterium disappeared, while bands representing Clostridium appeared. A second cycle of low/high alkalinity did not result in obvious changes to the bacterial community. These results indicate that the sulfidogenic bioreactor favored higher influent alkalinity and that the different functional microbial populations responded well to the alkalinity changes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2010.07.055 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Selenium and concomitant anions removal in a fixed bed bioreactor to satisfy drinking water regulations and subsequent stability check of selenium-laden biosludge.
Environ Res
December 2024
Department of Civil Engineering, IIT Guwahati, Guwahati, India.
This is the first successful report on selenium bio-attenuation to satisfy drinking water regulations as per Indian Standards (10 μg/L) in the presence of concomitant nitrate and sulfate from water sources utilizing a fixed bed bioreactor. The bioreactor was immunized with blended microbial culture and worked in downflow mode under anoxic conditions at 30 ± 2 °C for around 190 days under varying influent selenate (100-500 μg/L as selenium), nitrate (50 mg/L), sulfate concentrations (as per selenium removal) and necessary dose of acetic acid (as COD, a carbon source) in synthetic groundwater, operated at an empty bed contact time (EBCT) of 45-120 min. After supplying an adequate dosage of sulfate and alteration of EBCT, selenium was found to comply with drinking water regulations and nitrate was completely removed.
View Article and Find Full Text PDFDevelopment of a flow-cell bioreactor for immobilized sulfidogenic sludge characterization using electrochemical HS microsensors.
Chemosphere
June 2024
Department of Mining, Industrial and ICT Engineering (EMIT), Research Group on Intelligent and Sustainable Resources and Industries (RIIS), Manresa School of Engineering (EPSEM), Universitat Politècnica de Catalunya (UPC), Av. Bases de Manresa 61-73, 08242, Manresa, Spain. Electronic address:
The sulfate-reduction process plays a crucial role in the biological valorization of SO gases. However, a complete understanding of the sulfidogenic process in bioreactors is limited by the lack of technologies for characterizing the sulfate-reducing activity of immobilized biomass. In this work, we propose a flow-cell bioreactor (FCB) for characterizing sulfate-reducing biomass using HS microsensors to monitor HS production in real-time within a biofilm.
View Article and Find Full Text PDFElemental sulfur biorecovery from phosphogypsum using oxygen-membrane biofilm reactor: Bioreactor parameters optimization, metagenomic analysis and metabolic prediction of the biofilm activity.
Bioresour Technol
May 2024
College of Sustainable Agriculture and Environmental Sciences, Agrobioscience Program, Mohammed VI Polytechnic University, Benguerir 43150, Morocco. Electronic address:
This work investigated elemental sulfur (S) biorecovery from Phosphogypsum (PG) using sulfur-oxidizing bacteria in an O-based membrane biofilm reactor (MBfR). The system was first optimized using synthetic sulfide medium (SSM) as influent, then switched to biogenic sulfide medium (BSM) generated by biological reduction of PG alkaline leachate. The results using SSM had high sulfide-oxidation efficiency (98 %), sulfide to S conversion (∼90 %), and S production rate up to 2.
View Article and Find Full Text PDFA novel sulfidogenic process via sulfur reduction to remove arsenate in acid mine drainage: Insights into the performance and microbial mechanisms.
Water Res
May 2024
Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial International Joint Research Center on Urban Water Management and Treatment, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:
Biological sulfidogenic processes based on sulfate-reducing bacteria (SRB) are not suitable for arsenic (As)-containing acid mine drainage (AMD) treatment because of the formation of the mobile thioarsenite during sulfate reduction. In contrast, biological sulfidogenic processes based on sulfur-reducing bacteria (SRB) produce sulfide without pH increase, which could achieve more effective As removal than the SRB-based process. However, the reduction ability and toxicity tolerance of SRB to As remains mysterious, which may substantially affect the practical applicability of this process when treating arsenate (As(V))-containing AMD.
View Article and Find Full Text PDFIntegrated biological system for remediation and valorization of tannery wastewater: Focus on microbial communities responsible for methanogenesis and sulfidogenesis.
Bioresour Technol
March 2024
Applied Microbial and Health Biotechnology Institute, Cape Peninsula University of Technology, Symphony way, Bellville, Cape Town 7535, South Africa; Department of Chemical Engineering, Cape Peninsula University of Technology, Symphony way, Bellville, Cape Town 7535, South Africa. Electronic address:
Microbial communities in hybrid linear flow channel reactors and anaerobic sequencing batch reactors operated in series for remediation and beneficiation of tannery wastewater were assessed. Despite concurrent sulfidogenesis, more intensive pre-treatment in hybrid linear flow channel reactors reduced methanogenic inhibition usually associated with anaerobic digestion of tannery effluent and promoted efficiency (max 321 mLCH/gCOD, 59% biogas CH). Nitrification and biological sulfate reduction were key metabolic pathways involved in overall and sulfate reducing bacterial community selection, respectively, during pre-treatment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!