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Screening of in vivo activated genes in Enterococcus faecalis during insect and mouse infections and growth in urine. | LitMetric

AI Article Synopsis

  • - Enterococcus faecalis is normally found in the human gut and is harmless, but it can cause serious hospital infections due to its ability to become pathogenic.
  • - Researchers developed a Recombination-based In Vivo Expression Technology (R-IVET) to understand how E. faecalis transforms from a commensal organism to a dangerous pathogen, identifying 81 genes that are activated in vivo.
  • - Key findings include the significance of the ef_3196/7 operon and the gene ef_0377, both of which are crucial for the pathogenic capability of E. faecalis in models involving insects and mammals.

Article Abstract

Enterococcus faecalis is part of the commensal microbiota of humans and its main habitat is the gastrointestinal tract. Although harmless in healthy individuals, E. faecalis has emerged as a major cause of nosocomial infections. In order to better understand the transformation of a harmless commensal into a life-threatening pathogen, we developed a Recombination-based In VivoExpression Technology for E. faecalis. Two R-IVET systems with different levels of sensitivity have been constructed in a E. faecalis V583 derivative strain and tested in the insect model Galleria mellonella, during growth in urine, in a mouse bacteremia and in a mouse peritonitis model. Our combined results led to the identification of 81 in vivo activated genes. Among them, the ef_3196/7 operon was shown to be strongly induced in the insect host model. Deletion of this operonic structure demonstrated that this two-component system was essential to the E. faecalis pathogenic potential in Galleria. Gene ef_0377, induced in insect and mammalian models, has also been further analyzed and it has been demonstrated that this ankyrin-encoding gene was also involved in E. faecalis virulence. Thus these R-IVET screenings led to the identification of new E. faecalis factors implied in in vivo persistence and pathogenic potential of this opportunistic pathogen.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912369PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011879PLOS

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