Terephthalate (TA) is one of the top 50 chemicals produced worldwide. Its production results in a TA-containing wastewater that is treated by anaerobic processes through a poorly understood methanogenic syntrophy. Using metagenomics, we characterized the methanogenic consortium inside a hyper-mesophilic (that is, between mesophilic and thermophilic), TA-degrading bioreactor. We identified genes belonging to dominant Pelotomaculum species presumably involved in TA degradation through decarboxylation, dearomatization, and modified β-oxidation to H(2)/CO(2) and acetate. These intermediates are converted to CH(4)/CO(2) by three novel hyper-mesophilic methanogens. Additional secondary syntrophic interactions were predicted in Thermotogae, Syntrophus and candidate phyla OP5 and WWE1 populations. The OP5 encodes genes capable of anaerobic autotrophic butyrate production and Thermotogae, Syntrophus and WWE1 have the genetic potential to oxidize butyrate to CO(2)/H(2) and acetate. These observations suggest that the TA-degrading consortium consists of additional syntrophic interactions beyond the standard H(2)-producing syntroph-methanogen partnership that may serve to improve community stability.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105669 | PMC |
| http://dx.doi.org/10.1038/ismej.2010.125 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mangroves increased the mercury methylation potential in the sediment by producing organic matters and altering microbial methylators community.
Sci Total Environ
January 2025
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address:
Mangrove ecosystem has attracted global attention as a hotspot for mercury (Hg) methylation. Although numerous biotic and abiotic parameters have been reported to influence methylmercury (MeHg) production in sediments, the key factors determining the elevated MeHg levels in mangrove wetlands have not been well addressed. In this study, Hg levels in the sediments from different habitats (mudflats, mangrove fringe, and mangrove interior) in the Futian mangrove wetland were investigated, aiming to characterize the predominant factors affecting the MeHg production and distinguish the key microbial taxa responsible for Hg methylation.
View Article and Find Full Text PDFUtilizing conductive materials for reducing methane emissions in postharvest paddy rice soil microcosms.
Sci Total Environ
January 2025
Program of Sustainability in Biosystems, Institute of Agrifood Research and Technology (IRTA), Caldes de Montbui, Barcelona, Spain. Electronic address:
Paddy fields are a major anthropogenic source of global methane (CH) emissions, a powerful greenhouse gas (GHG). This study aimed at gaining insights of different organic and inorganic conductive materials (CMs) - biochar, fungal melanin, and magnetite - to mitigate CH emissions, and on their influence on key microbial populations, mimicking the postharvest season throughout the degradation of rice straw in microcosms under anaerobic conditions encompassing postharvest paddy rice soils from the Ebro Delta, Spain. Results showed that fungal melanin was the most effective CM, significantly reducing CH emissions by 29 %, while biochar amendment also reduced emissions by 10 %.
View Article and Find Full Text PDFTracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.
ISME Commun
January 2024
Otto-von-Guericke University Magdeburg, Bioprocess Engineering, Universitätsplatz 2, 39106 Magdeburg, Saxony-Anhalt, Germany.
A comprehensive understanding of microbial community dynamics is fundamental to the advancement of environmental microbiology, human health, and biotechnology. Metaproteomics, defined as the analysis of all proteins present within a microbial community, provides insights into these complex systems. Microbial adaptation and activity depend to an important extent on newly synthesized proteins (nP), however, the distinction between nP and bulk proteins is challenging.
View Article and Find Full Text PDFSynergy and competition during the anaerobic degradation of N-acetylglucosamine in a methane-emitting, subarctic, pH-neutral fen.
Front Microbiol
December 2024
Institute of Microbiology, Leibniz University Hannover, Hannover, Germany.
Peatlands are invaluable but threatened ecosystems that store huge amounts of organic carbon globally and emit the greenhouse gasses carbon dioxide (CO) and methane (CH). Trophic interactions of microbial groups essential for methanogenesis are poorly understood in such systems, despite their importance. Thus, the present study aimed at unraveling trophic interactions between fermenters and methanogens in a nitrogen-limited, subarctic, pH-neutral fen.
View Article and Find Full Text PDFIncreased methane production associated with community shifts towards Methanocella in paddy soils with the presence of nanoplastics.
Microbiome
December 2024
State Key Laboratory of Nutrient Use and Management, Key Laboratory of Plant-Soil Interactions, College of Resources and Environmental Sciences, Ministry of Education, National Academy of Agriculture Green Development, China Agricultural University, Beijing, 100193, China.
Background: Planetary plastic pollution poses a major threat to ecosystems and human health in the Anthropocene, yet its impact on biogeochemical cycling remains poorly understood. Waterlogged rice paddies are globally important sources of CH. Given the widespread use of plastic mulching in soils, it is urgent to unravel whether low-density polyethylene (LDPE) will affect the methanogenic community in flooded paddy soils.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!