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Selection of Reference Genes of Flower Development in .
Genes (Basel)
September 2024
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Article Synopsis
- RT-qPCR is used to identify suitable reference genes for accurate normalization in experiments, reducing errors.
- The study focuses on specific floral structures to select stable reference genes during developmental stages.
- Nine housekeeping genes were analyzed, with one emerging as the most stable for assessing flower development, aiding future research in this area.
Improving departmental psychological safety through a medical school-wide initiative.
BMC Med Educ
July 2024
Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, MI, USA.
Background: Psychological safety is a team-based phenomenon whereby group members are empowered to ask questions, take appropriate risks, admit mistakes, propose novel ideas, and candidly voice concerns. Growing research supports the benefits of psychological safety in healthcare and education for patient safety, learning, and innovation. However, there is a paucity of research on how to create psychological safety, especially within academic medicine.
View Article and Find Full Text PDFPredicting performance in attention by measuring key metabolites in the PCC with 7T MRS.
Sci Rep
July 2024
Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University, Aachen, Germany.
The posterior cingulate cortex (PCC) is a key hub of the default mode network and is known to play an important role in attention. Using ultra-high field 7 Tesla magnetic resonance spectroscopy (MRS) to quantify neurometabolite concentrations, this exploratory study investigated the effect of the concentrations of myo-inositol (Myo-Ins), glutamate (Glu), glutamine (Gln), aspartate or aspartic acid (Asp) and gamma-amino-butyric acid (GABA) in the PCC on attention in forty-six healthy participants. Each participant underwent an MRS scan and cognitive testing, consisting of a trail-making test (TMT A/B) and a test of attentional performance.
View Article and Find Full Text PDFEstablishing minimal clinically important differences for the Pemphigus Disease Area Index.
Br J Dermatol
October 2024
Department of Dermatology, St George Hospital, Sydney, NSW, Australia.
Background: Pemphigus is a rare autoimmune blistering disease with potentially life-threatening consequences. Establishing minimal clinically important differences (MCIDs) for disease severity scores like the Pemphigus Disease Area Index (PDAI) is crucial for assessing treatment efficacy.
Objectives: To calculate MCIDs for both improvement and deterioration in PDAI scores in patients with pemphigus vulgaris (PV) and pemphigus foliaceus (PF), using the anchor-based method.
Keypoint-MoSeq: parsing behavior by linking point tracking to pose dynamics.
Nat Methods
July 2024
Department of Neurobiology, Harvard Medical School, Boston, MA, USA.
Keypoint tracking algorithms can flexibly quantify animal movement from videos obtained in a wide variety of settings. However, it remains unclear how to parse continuous keypoint data into discrete actions. This challenge is particularly acute because keypoint data are susceptible to high-frequency jitter that clustering algorithms can mistake for transitions between actions.
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