Objective: To define the structure of a water-soluble compound (SY-A) extracted from Dioscorea zingiberensis and investigate its protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion (I/R) injured.
Methods: The SY-A was isolated from Dioscorea zingiberensis by utilizing column chromatograph and its structure was identified by 1H-NMR, 13C-NMR, 135DEPT. Neonatal rat cardiomyocytes were cultured and divided into 5 groups randomly. Then a model of ischemic/reperfusion (I/R) was established. Cells were divided into control group, simulated ischemia/reperfusion group (I/R group), I/R + SY-A (50 mg/L) group, I/ R + SY-A (100 mg/L) group and I/R + SY-A (150 mg/L) group. The levels of malondialdehyde and Creatine Kinase were measured. The cell viability was determined by MTT assay.
Results: The structure of SY-A was identified as 4-methoxybenzoic acid-2-O-glucopyranoside. Compared with I/R group, the levels of LDH and CK were decreased significantly in the SY-A group, and the cell viability was increased obviously.
Conclusion: Extractive of Dioscorea zingiberensis showed protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion injured.
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