Hyperspectral imaging (400-720 nm) and discriminate analysis were investigated for the detection of normal and diseased cucumber leaf samples with powdery mildew (Sphaerotheca fuliginea), angular leaf spot (Pseudomopnas syringae), downy mildew (Pseudoperonospora cubensis), and brown spot (Corynespora cassiicola). A hyperspectral imaging system was es tablished to acquire and pre-process leaf images, as well as to extract leaf spectral properties. Owing to the complexity of the original spectral data, stepwise discriminate and canonical discriminate were executed to reduce the numerous spectral information, in order to decrease the amount of calculation and improve the accuracy. By the stepwise discriminate we selected 12 optimal wavelengths from the original 55 wavelengths, and after the canonical discriminate, the 55 wavelengths were reduced to 2 canonical variables. Then the discriminate models were developed to classify the leaf samples. The result shows that the stepwise discriminate model achieved classification accuracies of 100% and 94% for the training and testing sets, respectively. For the canonical model, the classification accuracies for the training and testing sets were both 100%. These results indicated that it is feasible to identify and classify cucumber diseases using hyperspectral imaging technology and discriminate analysis. The preliminary study, which was done in a closed room with restrictions to avoid interference of the field environment, showed that there is a potential to establish an online field application in cucumber disease detection based on visible spectroscopy.
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Sci Rep
December 2024
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People's Republic of China.
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December 2024
College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot, China.
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State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, 712100, China; Key Laboratory of Wheat Biology and Genetic Improvement on Northwestern China, Ministry of Agriculture and Rural Affairs, Xianyang, 712100, China. Electronic address:
Photosynthesis drives crop growth and production, and strongly affects grain yields; therefore, it is an ideal trait for wheat drought resistance breeding. However, studies of the negative effects of drought stress on wheat photosynthesis rates have lacked accurate evaluation methods, as well as high-throughput techniques. We investigated photosynthetic capacity under drought stress in wheat varieties with varying degrees of drought stress resistance using hyperspectral and chlorophyll fluorescence (ChlF) imaging data.
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School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
The levels of capsaicin (CAP) and hydroxy-α-sanshool (α-SOH) are crucial for evaluating the spiciness and numbing sensation in spicy hotpot seasoning. Although liquid chromatography can accurately measure these compounds, the method is invasive. This study aimed to utilize hyperspectral imaging (HSI) combined with machine learning for the nondestructive detection of CAP and α-SOH in hotpot seasoning.
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Ultraviolet (UV) hyperspectral imaging shows significant promise for the classification and quality assessment of raw cotton, a key material in the textile industry. This study evaluates the efficacy of UV hyperspectral imaging (225-408 nm) using two different light sources: xenon arc (XBO) and deuterium lamps, in comparison to NIR hyperspectral imaging. The aim is to determine which light source provides better differentiation between cotton types in UV hyperspectral imaging, as each interacts differently with the materials, potentially affecting imaging quality and classification accuracy.
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