The whole antral follicles were isolated from porcine ovaries and classified as follows: healthy follicles (HF), early atretic follicles (EF) and progressed atretic follicles (PF). The isolated porcine follicles were used for routine histological section and HE staining after examination by eyesight. Morphological research shows that the accuracy rate of eyesight examination for HF is 92%. Healthy follicles were chosen for further experiment and divided into 3 groups: large follicles (greater than 5 mm in diameter), medium follicles (3-5 mm in diameter) and small follicles (less than 3 mm in diameter). All follicles were cultured for 8, 16 and 24 h, respectively and the apoptosis of of their granulosa cells were examined by Annexin V-FITC/PI double-labeling. It showed that the total apoptotic rate of granulosa cells derived from cultured follicles could reach over 70% at 8 h after culture and be 81.1% - 94.6% at 24 h after culture. Granulosa cells from groups were collected at 0, 8, 16, 24, 48 and 72 h after culture without serum and used for the examination of expression of FasL and Fas mRNA with real time PCR SYBRgreen method. The expression level of FasL mRNA of granulosa cells from different size of follicles increased with culture time and reached the highest level at 24 h after culture (P<0.05). Expression level of FasL mRNA of granulosa cells from small follicles was higher than those from large and medium follicles. There exists no difference for expression level of Fas mRNA of granulosa cells among groups before culture but significantly increased at 8 h after culture and reached the highest level at 48 h after culture. It showed in the present experiment that the follicular culture system without serum used could effectively induce the apoptosis of follicular granulosa cells. Cell apoptosis is the main cause of follicular atresia, the degree of which varied with the size of follicles. Small follicles seemed to be easier atretic than medium and large follicles.
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http://dx.doi.org/10.3724/SP.J.1141.2010.03268 | DOI Listing |
Reprod Domest Anim
January 2025
Tianzhu County Animal Husbandry Technology Extension Station, Tianzhu, Gansu, China.
Granulosa cells (GCs) are pivotal in the development of ovarian follicles, serving not only as supportive cells but also as the primary producers of steroid hormones. The proliferation of these cells and the synthesis of steroid hormones are crucial for follicular development and atresia. In our study, GCs were isolated using follicular fluid aspiration and subsequently identified through immunofluorescence.
View Article and Find Full Text PDFJ Dairy Sci
January 2025
Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Institute, Rishon LeZion, Israel. Electronic address:
Activation of the endocannabinoid system (ECS) elicits negative effects on the reproductive system in mammals. Omega-3 (n-3) fatty acid (FA) supplementation lowers ECS activation and has anti-inflammatory effects. Thus, we hypothesized that supplementing cows with n-3 FA will downregulate components of the ECS and immune system in preovulatory follicles and in the endometrium.
View Article and Find Full Text PDFGranulosa tumors are rare tumors arising from the cells of the sexual cord and stroma of the ovary. They account for 5% of ovarian cancers and 70% of stromal cancers of the sex cords. Retroperitoneal tumors (RPTs) are also rare and develop in the retroperitoneal and subperitoneal space.
View Article and Find Full Text PDFReprod Sci
January 2025
Department of Neurosurgery, First Affiliated Hospital of Xiamen University; School of Medicine, Xiamen University, Xiamen, China.
Purpose: To explore the impact of high body mass index (BMI) on the embryo quality and clinical outcomes of polycystic ovary syndrome (PCOS) patients, and the possible genes involved.
Methods: Patients who underwent in-vitro fertilization (IVF) treatment and embryo transfer in our center from November 2014 to September 2023, were divided into low BMI PCOS (LBP) group, high BMI PCOS (HBP) group, and high BMI control (HBC) group. Transcriptome sequencing was performed in eight PCOS patients' granulosa cells (GCs).
J Hazard Mater
January 2025
Centre of Reproductive Medicine, Department of Obstetrics and Gynaecology, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, Tiexi District, Shenyang, Liaoning 110022, PR China; Key Laboratory of Reproductive Dysfunction Disease and Fertility Remodelling of Liaoning Province, Shenyang, Liaoning 110022, PR China. Electronic address:
Sulfur dioxide (SO) is a contributor to air pollution. Human evidence has demonstrated an association between SO exposure and diminished ovarian reserve. The toxicity of SO is mainly attributed to its derivatives, bisulfite and sulfite, which have a variety of adverse effects on both human health and the environment, yet have been widely used as additives in food processing and transportation.
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