AI Article Synopsis
- The study explores the interaction between the poxA gene, a paralog of lysyl tRNA-synthetase, and the yjeK gene in Salmonella enterica, which affects the bacteria's virulence and resistance to stress.
- Both poxA and yjeK mutants exhibit similar phenotypic traits, including reduced virulence in mice and enhanced respiration under limited nutrient conditions, as well as increased sensitivity to various growth inhibitors.
- PoxA's function involves the posttranslational modification of elongation factor P (EF-P), which is crucial for regulating protein synthesis in bacteria, resembling a similar process in eukaryotes with eIF5A.
Article Abstract
We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913146 | PMC |
| http://dx.doi.org/10.1016/j.molcel.2010.06.021 | DOI Listing |
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Article Synopsis
- The study explores the interaction between the poxA gene, a paralog of lysyl tRNA-synthetase, and the yjeK gene in Salmonella enterica, which affects the bacteria's virulence and resistance to stress.
- Both poxA and yjeK mutants exhibit similar phenotypic traits, including reduced virulence in mice and enhanced respiration under limited nutrient conditions, as well as increased sensitivity to various growth inhibitors.
- PoxA's function involves the posttranslational modification of elongation factor P (EF-P), which is crucial for regulating protein synthesis in bacteria, resembling a similar process in eukaryotes with eIF5A.
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