AI Article Synopsis
Article Abstract
Hydrolysis of TA photoproduct leads to two derivatives presenting different formation kinetic profiles depending on the oligomer content. The formation efficiency of TA photoproducts in UV-C-irradiated DNA slightly exceeds the formation of the trans,syn cyclobutane pyrimidine dimer at TT sites.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja1023173 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dynamics and mechanism of DNA repair by a bifunctional cryptochrome.
Proc Natl Acad Sci U S A
December 2024
Center for Ultrafast Science and Technology, School of Chemistry and Chemical Engineering, School of Physics and Astronomy, Zhang Jiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai 200240, China.
Photolyase and cryptochrome belong to a group of structurally similar flavoproteins but with two distinct functions of DNA repair as a photoenzyme and signal transduction as a photoreceptor, respectively, under blue-light illumination. Here, we studied a recently discovered bifunctional cryptochrome (CraCRY) with focus on its repair of UV-induced pyrimidine-pyrimidone (6-4) photoproduct (6-4PP). We used femtosecond spectroscopy and site-directed mutagenesis to map out the critical elementary steps by following the dynamics of initial reactants, various intermediates, and final products.
View Article and Find Full Text PDFPhotochemical Hydroxyl Group Abstraction from -Hydroxypyridine-2(1)-thione Isolated in a Solid Hydrogen Matrix: Photogeneration of 2-Mercaptopyridine.
Molecules
November 2024
Institute of Physics, Polish Academy of Sciences, Al. Lotnikow 32/46, 02-668 Warsaw, Poland.
Monomers of -hydroxypyridine-2(1)-thione were isolated in low-temperature matrices of solid normal hydrogen (n-H). The matrix-isolated compound was irradiated with UV-B (λ = 305 nm) or UV-A (λ > 360 nm) light. Upon such irradiation, the initial form of -hydroxypyridine-2(1)-thione was completely consumed and converted into photoproducts.
View Article and Find Full Text PDFAlterations in the mechanical properties of single dsDNA molecules, bare or cell-encapsulated, upon exposure to UVA-only radiation and sunlight.
J Photochem Photobiol B
December 2024
School of Biological Sciences, Indian Association for the Cultivation of Science, Kolkata, West Bengal, India. Electronic address:
Exposure to ultraviolet radiation, which leads to the formation of mutagenic and cytotoxic DNA lesions such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4 PPs), can be potentially fatal. The way UVA forms DNA lesions and alters DNA topology and mechanics is still unclear, unlike the cases of UVC and UVB. Herein, Atomic Force Microscopy (AFM) and AFM-based Force Spectroscopy (AFS) have been employed to investigate the topological and mechanical properties of single DNA molecules, bare or E.
View Article and Find Full Text PDFThe photoreactivation of 6 - 4 photoproducts in chloroplast and nuclear DNA depends on the amount of the Arabidopsis UV repair defective 3 protein.
BMC Plant Biol
July 2024
Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland.
Background: 6 - 4 photoproducts are the second most common UV-induced DNA lesions after cyclobutane pyrimidine dimers. In plants, they are mainly repaired by photolyases in a process called photoreactivation. While pyrimidine dimers can be deleterious, leading to mutagenesis or even cell death, 6 - 4 photoproducts can activate specific signaling pathways.
View Article and Find Full Text PDFOn the Use of the Intrinsic DNA Fluorescence for Monitoring Its Damage: A Contribution from Fundamental Studies.
ACS Omega
June 2024
Université Paris-Saclay, CNRS, Institut de Chimie Physique, UMR8000, 91405 Orsay, France.
The assessment of DNA damage by means of appropriate fluorescent probes is widely spread. In the specific case of UV-induced damage, it has been suggested to use the emission of dimeric photoproducts as an internal indicator for the efficacy of spermicidal lamps. However, in the light of fundamental studies on the UV-induced processes, outlined in this review, this is not straightforward.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!