AI Article Synopsis
- FFPE tissues serve as the main method for storing patient samples, particularly in cancer research.
- The study used targeted proteomic analyses to compare protein levels in epithelial cells from both endometrial carcinoma and normal endometrial tissues, finding similar results for specific biomarkers.
- Alongside pyruvate kinase isoform M2 (PK-M2) and polymeric Ig receptor, other proteins were detected, but only inconsistently or at too low levels for reliable measurement.
Article Abstract
Formalin-fixed paraffin-embedded (FFPE) tissues are the primary and preferred medium for archiving patients' samples. Here we demonstrate relative quantifications of protein biomarkers in extracts of laser microdissected epithelial cells from FFPE endometrial carcinoma tissues versus those from normal proliferative endometria by means of targeted proteomic analyses using LC-multiple reaction monitoring (MRM) MS with MRM Tags for Relative and Absolute Quantitation (mTRAQ) labeling. Comparable results of differential expressions for pyruvate kinase isoform M2 (PK-M2) and polymeric Ig receptor were observed between analyses on laser microdissected epithelial cells from FFPE tissues and corresponding homogenates from frozen tissues of the same individuals that had previously been analyzed and reported. We also identified PK-M2 in the normal proliferative phase of the endometrium. Other biomarkers in addition to PK-M2 and polymeric Ig receptor were also observed but not consistently and/or were at levels below the threshold for quantification.
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| http://dx.doi.org/10.1002/pmic.201000082 | DOI Listing |
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