AI Article Synopsis
- The study examines how La3+ affects K+ channel currents in horseradish cells using various advanced techniques.
- It discovers that La3+ reduces these currents by forming bonds with the K+ channel protein, altering its structure.
- This research enhances our understanding of how high-valence metal ions interact with channel proteins in both plant and animal cells.
Article Abstract
The current of the outward K+ channel in the cell of horseradish treated with La3+ and the direct interaction between La3+ and the K+ channel protein were investigated using the whole-cell patch-clamp technique, molecular dynamics simulation, and quantum chemistry calculation methods. It was found for the first time that La3+ decreases the current of the K+ channel in the horseradish mesophyll cell. The decrease results from the formation of a coordination bond and hydrogen bond between La3+ and the K+ channel protein in the plasma membrane. The direct interaction destroys the native structure of the K+ channel protein, disturbing the function of the K+ channel protein in the cells. The results can provide the theoretical foundation for understanding the interaction between metal ions (especially high-valence metal ions) and the channel protein in organisms, including animal and plant cells.
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| http://dx.doi.org/10.1007/s00775-010-0688-0 | DOI Listing |
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