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Probing protease activity by single-fluorescent-protein nanocapsules. | LitMetric
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Probing protease activity by single-fluorescent-protein nanocapsules.

Zhen Gu Anuradha Biswas Kye-Il Joo Biliang Hu Pin Wang Yi Tang

Chem Commun (Camb)

Department of Chemical and Biomolecular Engineering, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA.

Published: September 2010

We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.

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 Source
http://dx.doi.org/10.1039/c0cc01439gDOI Listing

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