In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.molbiopara.2010.07.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synergistic Effects of Anti-echinococcosis Drug Candidates Combined With Atovaquone in Culture Assays and Mice With Primary Infections of Echinococcus multilocularis.
Cureus
November 2024
Department of Pediatrics, Teikyo University School of Medicine, Tokyo, JPN.
Background Alveolar echinococcosis (AE) is a fatal zoonotic disease distributed mainly in the Northern Hemisphere. At present, its curative treatment relies on surgery, and the development of effective drugs is needed. We previously demonstrated the anti-echinococcal effect of atovaquone (ATV) as a mitochondrial complex III inhibitor in both in vitro and in vivo experiments.
View Article and Find Full Text PDFRisk factors associated with human echinococcosis: a systematic review and meta-analysis.
Front Vet Sci
November 2024
Scientific Affairs Department, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq.
Introduction: Echinococcosis is a widespread zoonotic disease caused by tapeworms of the Echinococcus genus, manifesting in mature or larval forms. Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are the primary types affecting humans, linked, respectively, to and . This study is a systematic review and meta-analysis of the risk factors associated with CE and AE in humans.
View Article and Find Full Text PDF[Hepatic T cell subtypes and functional analysis among alveolar echinococcosis patients using single-cell RNA sequencing].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
November 2024
Qinghai Provincial People's Hospital, Clinical Research Institute of Hydatid Disease, Xining, Qinghai 810007, China.
Objective: To investigate T cell subtypes and their functions in liver immune microenvironments among patients with alveolar echinococcosis (AE) using single-cell RNA sequencing (scRNA-seq).
Methods: Four AE patients that were admitted to Qinghai Provincial People's Hospital in 2023 for hepatic surgery for the first time were enrolled, and liver specimens were sampled 1 cm (peri-lesion, PL group) and > 5 cm from AE lesions (distal lesion, DL group) among each patient. Finally, a total of eight liver specimens were sampled from four AE patients for scRNA-seq analysis.
Current status of drug therapy for alveolar echinococcosis.
World J Hepatol
November 2024
Department of Hepatobiliary and Pancreatic Surgery, Qinghai Province Research Key Laboratory for Echinococcosis, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China.
Article Synopsis
- * Current treatment primarily involves surgery or lifelong use of anti-AE medications like albendazole and mebendazole, which can manage the disease but are not curative and may cause harmful side effects.
- * New potential therapies are being explored, including synthetic and natural compounds, which aim to improve effectiveness and reduce side effects by targeting the parasitic metabolism and structure, with ongoing research to refine these treatment options.
Resolution of infertility following hydatid cyst removal: a case report.
Ann Med Surg (Lond)
November 2024
Al-Ahli Hospital, Hebron, State of Palestine.
Introduction And Importance: Human echinococcosis, also known as 'hydatid cyst,' constitutes a zoonotic parasitic disease attributed to or . Primary afflictions occur in the liver and lungs, whilst the implication of alternative organs remains infrequent. The clinical presentation can vary, and large cysts may cause compression symptoms and complications.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!