AI Article Synopsis
- The study aimed to assess the biocompatibility of a new diatomite-based ceramic by examining its effects on L929 cells in comparison to other prosthodontic materials.
- Various concentrations of the ceramic and other materials were tested on a cell line, with apoptosis and cell cycle progression analyzed using flow cytometry and specific staining methods.
- Results indicated that the new ceramic had no significant effect on cell apoptosis and demonstrated low cytotoxicity, aligning with the requirements for clinical biocompatibility.
Article Abstract
Objective: To evaluate a new type of diatomite-based machinable ceramic biocompatibility by studying its induced apoptosis on L929 cell in contrasted with other prosthodontics materials.
Methods: Cell line was treated with extracting liquid containing different concentrations of diatomite-based machinable ceramic and other materials. Flow cytometry tested cell cycle progression and induced cell apoptosis. Annexin V-FITC/PI apoptosis staining kit quantitative detected cell death patterns. The expression of Bcl-2 and Bax mRNA were determined by reverse transcription-polymerase chain reaction.
Results: The experimental groups had no special influence on cell cycle. Apoptosis rates of the new ceramic closed to the negative group (P > 0.05). The apoptosis rate of resin was the highest, and the cell necrosis level of resin was increased, which had significant difference to the new ceramic (P < 0.05). The Bcl-2 and Bax mRNA levels of the new ceramic and the negative group were closed to each other, which had no statistical significance (P > 0.05).
Conclusion: The new diatomite-based machinable ceramic has no apparent cytotoxicity, which is consistent with the clinical application of the basic requirements of biocompatibility.
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