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A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events. | LitMetric

AI Article Synopsis

  • Prestin is a crucial motor protein in outer hair cells that helps amplify sound in the cochlea, and its expression begins before mice are able to hear.
  • Researchers created a special cell line to study how prestin matures functionally and how it works in relation to other biochemical factors.
  • They noticed a two-phase growth pattern in prestin's charge density, with one phase indicating a dominant-negative effect from a smaller monomer, and they also observed changes similar to those in developing outer hair cells.

Article Abstract

Prestin is the motor protein within the lateral membrane of outer hair cells (OHCs), and it is required for mammalian cochlear amplification. Expression of prestin precedes the onset of hearing in mice, and it has been suggested that prestin undergoes a functional maturation within the membrane coincident with the onset of hearing. We have developed a tetracycline-inducible prestin-expressing cell line that we have used to model prestin's functional maturation. We used prestin's voltage-dependent nonlinear charge movement (or nonlinear capacitance) as a test of function and correlated it to biochemical measures of prestin expressed on the cell surface. An initial stage of slow growth in charge density is accompanied by a rapid increase in our estimate of charge carried by an individual motor. A rapid growth in charge density follows and strongly correlates with an increasing ratio between an apparently larger and smaller monomer, suggesting that the latter exerts a dominant-negative effect on function. Finally, there is a gradual depolarizing shift in the voltage of peak capacitance, similar to that observed in developing OHCs. This inducible system offers many opportunities for detailed studies of prestin.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774197PMC
http://dx.doi.org/10.1152/ajpcell.00182.2010DOI Listing

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