AI Article Synopsis
- A new HPLC method was developed using a photodiode array detector to measure five bioactive compounds in Cortex Phellodendri, specifically limonin, phellodendrine, jatrorrhizine, palmatine, and berberine.
- The analysis employed an Agilent Diamonsil C18 column with a specific gradient and flow rate, and it successfully showed linear calibration curves for the compounds across various concentration ranges.
- The method demonstrated high recovery rates and low variability, proving it to be efficient, sensitive, and reliable for studying the bioactive components in the samples from different geographical areas.
Article Abstract
An HPLC method combined with a photodiode array detector was developed for quantitative determination of five bioactive compounds that belong to two subclasses, including limonin, phellodendrine, jatrorrhizine, palmatine, and berberine in Cortex Phellodendri. The analysis was performed on an Agilent Diamonsil C18 column (4.6 x 250 mm, 5 microm) using a gradient of acetonitrile and 0.3% aqueous diethylamine phosphate (v/v), a flow rate of 0.8 mL/min, and a detection wavelength of 220 nm. The calibration curve was linear over the range of 2.5-100.0 microg/mL for both phellodendrine and jatrorrhizine, 5.0-200.0 microg/mL for palmatine, and 7.5-300.0 microg/mL for both berberine and limonin. The average recoveries ranged from 97.56 to 102.53% with RSD < or = 1.00%. Samples from different geographical locations were analyzed to evaluate the applicability of the established method, and the results indicated that the method was efficient, sensitive, and reliable for determining limonin and four alkaloids in Cortex Phellodendri.
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