NAGase (EC.3.2.1.52) from crustaceans has the important roles in immunity, molting and digestion of chitinous foods. In this paper, the effects of citric acid on the activity of NAGase from Litopenaeus vannamei for the hydrolysis of pNP-NAG have been studied. The results showed that appropriate concentrations of citric acid could lead to reversible inhibition on NAGase and IC(50) was estimated to be 5.00 +/- 0.35 mM. Using the plots of Lineweaver-Burk, the inhibition of NAGase by citric acid belongs to competitive type, the inhibitory equilibrium constant for citric acid binding with free NAGase, K(I), is 3.26 +/- 0.25 mM. The inhibitory kinetics of citric acid on NAGase in the appropriate concentrations of citric acid has been studied using the kinetic method of substrate reaction. The time course of NAGase for the hydrolysis of pNP-NAG in the presence of different concentrations of citric acid showed that at each citric acid concentration, the rate decreased with increasing time until a straight line was approached. The results show that the inhibition of NAGase by citric acid is a slow, reversible reaction with fractional remaining activity. The microscopic rate constants are determined for the reaction on citric acid with NAGase.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2010.07.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antimicrobial activity and synergistic effect of phage-encoded antimicrobial peptides with colistin and outer membrane permeabilizing agents against .
PeerJ
December 2024
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok, Thailand.
Background: poses a significant public health threat. Phage-encoded antimicrobial peptides (AMPs) have emerged as promising candidates in the battle against antibiotic-resistant .
Methods: Antimicrobial peptides from the endolysin of bacteriophage were designed from bacteriophage vB_AbaM_PhT2 and vB_AbaAut_ChT04.
Comprehensive evaluation of sodium dichloroisocyanurate (NaDCC) tablets as a novel solid-state alternative to conventional membrane cleaning agents in gravity-driven filtration systems.
Chemosphere
December 2024
Department of Environmental Research, Korea Institute of Civil Engineering and Building Technology, Gorang-Daero 283, Ilsanseo-Gu, Goyang, Gyeonggi 10223, Republic of Korea; Department of Civil and Environment Engineering, University of Science and Technology (UST), 217 Gajeong-Ro, Yuseong-Gu, Daejeon, 34113, Republic of Korea. Electronic address:
Gravity-driven membrane (GDM) systems are increasingly recognized as sustainable and energy-efficient solutions for decentralized water treatment. However, membrane fouling, particularly by organic matter, remains a significant operational challenge, necessitating regular chemical cleaning to maintain performance. The present study was undertaken to investigate the cleaning efficiency of sodium dichloroisocyanurate (NaDCC) tablets, a novel solid-state alternative to conventional liquid cleaning agents such as sodium hypochlorite (NaOCl), sodium lauryl sulfate (SLS), acetic acid, and citric acid.
View Article and Find Full Text PDFInvolvement of nitrate reductase in nitric oxide generation and in the induction of acclimation responses to phosphorus restriction in soybean plants.
Plant Sci
December 2024
Instituto de Fisiología Vegetal (INFIVE CCT CONICET La Plata), Universidad Nacional de La Plata (UNLP) y Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Diagonal 113 Nº 495 (1900) La Plata, Argentina. Electronic address:
Nitrate reductase (NR) is an essential enzyme because of its role in nitrogen metabolism and in key signaling events through the generation of the reactive nitrogen species, nitric oxide (NO). In this work, we evaluated changes in endogenous NO levels during the onset of P-restriction in soybean plants (Glycine max), focusing on the possible pathways involved in its generation, namely NR and NO synthase like activity, NOS, and the subsequent role of NR during low P-acclimation. During the first 96h of P-starvation NO levels increased in the leaves.
View Article and Find Full Text PDFCitric acid is more effective than sodium thiosulfate in chelating calcium in a dissolution model of calcinosis.
Sci Rep
December 2024
Division of Musculoskeletal and Dermatological Sciences, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester and Northern Care Alliance NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK.
Calcinosis cutis affects 20-40% of patients with systemic sclerosis. This study tests the hypothesis that calcium-chelating polycarboxylic acids can induce calcium dissolution without skin toxicity or irritancy. We compared citric acid (CA) and ethylenediaminetetraacetic acid (EDTA) to sodium thiosulfate (STS) for their ability to chelate calcium in vitro using a pharmaceutical dissolution model of calcinosis (hydroxyapatite (HAp) tablet), prior to evaluation of toxicity and irritancy in 2D in vitro skin models.
View Article and Find Full Text PDFWood-Derived Hydrogels for Osteochondral Defect Repair.
ACS Nano
December 2024
Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Repairing cartilage tissue is a serious global challenge. Herein, we focus on wood skeletal structures that are highly porous for cell penetration yet have load-bearing strength, and aim to synthesize wood-derived hydrogels with the ability to regenerate cartilage tissues. The hydrogels were synthesized by wood delignification and the subsequent intercalation of citric acid (CA), which is involved in tricarboxylic acid cycles and essential for energy production, and -acetylglucosamine (NAG), which is a cartilage glycosaminoglycan, among cellulose microfibrils.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!