The present study was conducted to assess the effects of effluent from a wastewater treatment plant (WWTP) on goldfish in early life stage. Fertilized eggs of the goldfish were exposed to a serial of graded concentrations of the effluent for 30 d. The hatching and survival rates of goldfish were decreased and the freaky rate was elevated with increased effluent concentrations. Additionally, the growth of fry was inhibited, and both body length and body weight were significantly decreased at > or =20% of effluent (p < 0.05). vitellogenin (Vtg) in fry was induced by all exposure concentrations. However, concentration dependence was not apparent. The highest Vtg induction was observed at the exposure of 20% effluent, whereas the induction effects of Vtg were decreased at > 20% of effluent. After a period of culture in clean water, both body length and body weight of fish were significantly increased, and Vtg levels were significantly decreased (p < 0.05). The result demonstrates that the exposure of WWTP effluents in early life stage can cause a risk of fish feminization. Goldfish seem to be suitable in assessing environmental impact of endocrine-disrupting chemicals due to their sensitivity in early life stage.
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Wiley Interdiscip Rev RNA
January 2025
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, People's Republic of China.
Life was originated from inorganic world and had experienced a long period of evolution in about 3.8 billion years. The time for emergence of the pioneer creations on Earth is debatable nowadays, and how the scenario for the prebiotic molecular interactions is still mysterious.
View Article and Find Full Text PDFJ Clin Psychiatry
January 2025
Department of Psychiatry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India, Department of Clinical Psychopharmacology and Neurotoxicology, National Institute of Mental Health and Neurosciences, Bangalore, India
Cannabis use during pregnancy is increasing; the study of adverse outcomes in cannabis-exposed pregnancies is therefore important. Previous articles in this series described increased risks of maternal adverse outcomes, fetal adverse outcomes, birth defects in newborns, and autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) in childhood. This article examines neuropsychiatric adverse outcomes in offspring gestationally exposed to cannabis.
View Article and Find Full Text PDFActa Orthop
January 2025
Spine Unit, Department of Orthopaedic Surgery, Rigshospitalet, Copenhagen, Denmark.
Background And Purpose: Treatment of idiopathic scoliosis in childhood aims to prevent curve progression. It is generally accepted that curves > 50° have the highest risk of progression, but less well described is what happens with mild to moderate curves. The aim of this study was to assess long-term curve progression and health-related quality of life (HRQoL) and compare thoracic and thoracolumbar/lumbar (TL/L) curves.
View Article and Find Full Text PDFCent Eur J Public Health
December 2024
Institute of Hygiene, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic.
Objectives: This study aimed to determine the metabolic syndrome (MS) prevalence in a sample of adolescents, to calculate their continuous metabolic syndrome scores, and to determine the associations of continuous metabolic syndrome score with overweight/obesity and selected cardiometabolic and lifestyle factors.
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Since microRNAs (miRNAs) serve as markers for early cancer diagnosis, it is crucial to develop a novel biosensor to detect miRNAs quickly, sensitively and selectively. Hence, we developed a fluorescence biosensor based on target miRNA-initiated rolling circle amplification (RCA) to generate RCA products with multiple tandem catalytic hairpin DNA templates that trigger primer exchange reactions (PER) which extend short single-strand DNA (ssDNA) primers into long ssDNA. Subsequently, the long ssDNA activates the -cleavage activity of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system to cleave a fluorescent reporter chain, enabling ultrasensitive detection of miRNAs through the output fluorescence signal.
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